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| 丹参酮ⅡA诱导人鼻咽癌CNE1细胞凋亡 |
| 赵坚1,2,许青3*,孙健4,徐晓春3 |
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| (1. 上海中医药大学附属普陀医院耳鼻喉科,上海 200062;2. 同济大学医学院,上海 200092;3. 同济大学附属第十人民医院肿瘤及放射治疗科,上海 200072;4. 上海中医药大学附属普陀医院中心实验室,上海 200062) |
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| 摘要: |
| 目的研究丹参酮ⅡA(TanshinoneⅡA,TanⅡA)对人鼻咽癌CNE1细胞诱导凋亡及肿瘤相关基因Bcl-2和COX-2启动子表达的影响。方法不同浓度的TanⅡA体外干预CNE1细胞,用MTT比色法检测TanⅡA对CNE1细胞增殖的抑制作用,流式细胞术检测CNE1细胞凋亡、细胞周期及抗凋亡基因Bcl-2的表达,双荧光素酶法检测CNE1细胞COX-2启动子的活性。结果经TanⅡA干预后,CNE1细胞生长出现抑制,抑制率与TanⅡA浓度及作用时间呈依赖效应,差异有统计学意义(P<0.01)；TanⅡA组细 |
| 关键词: 鼻咽肿瘤 丹参酮ⅡA 细胞凋亡 细胞周期 环氧化酶2 Bcl-2 |
| DOI：10.3724/SP.J.1008.2011.0879 |
| 投稿时间：2011-04-25修订日期：2011-07-07 |
| 基金项目: |
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| Tanshinone ⅡA induces apoptosis in human nasopharyngeal carcinoma CNE1 cells |
| ZHAO Jian1,XU Qing3*,SUN Jian4,XU Xiao-chun3 |
| (1. Department of Otorhinolaryngology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China;2. School of Medicine, Tongji University, Shanghai 200092, China;3. Department of Chemoradiotherapy, The Tenth People’s Hospital of Shanghai, Tongji University, Shanghai 200072, China;4. Central Laboratory, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China) |
| Abstract: |
| ObjectiveTo explore the effect of anshinoneⅡA(Tan ⅡA) on induction of apoptosis in human nasopharyngeal carcinoma CNE1 cells and on expression of Bcl-2 gene and COX-2 promoter. MethodsCNE1 cells were cultured in vitro and treated with various concentratio |
| Key words: nasopharyngeal neoplasms tanshinone apoptosis cell cycle cyclooxygenase 2 Bcl-2 |
