| 摘要: |
| 目的检测头颈鳞癌标本,寻找与头颈鳞癌转移高度相关的基因。方法采用单核苷酸多态性基因芯片技术,检测80例临床头颈鳞癌标本,包括舌癌17例,口腔癌13例,口咽癌14例,下咽癌36例。在随访期内,将发生远处转移的39例患者标本作为试验组,将未发生远处转移的41例患者标本作为对照组,利用Cox回归分析基因单核苷酸多态性对肿瘤远处转移的影响。结果单核苷酸多态性检测发现,在位于11q13的区域出现了一个基因组增多所致的独特的峰值。在这个基因组内,Cox回归分析显示,SHANK2基因的rs9651738位点的T等位基因 |
| 关键词: 鳞状细胞癌 头颈部肿瘤 基因芯片 单核苷酸多态性 肿瘤转移 |
| DOI：10.3724/SP.J.1008.2011.0713 |
| 投稿时间：2011-04-26修订日期：2011-05-27 |
| 基金项目: |
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| Relationship between single nucleotide polymorphism in genome of head and neck squamous cell carcinoma tissue and tumor metastasis |
| LI Ya-dong1,Ali Gowhere2,HONG Su-ling3* |
| (1. Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China;2. Centre Regional de Lutte Contre le Cancer Paul Strauss, 3 rue Porte de l’Hopital, Strasbourg 67085, France) |
| Abstract: |
| Objective: To find the genes correlated with the metastasis of head and neck squamous cell carcinoma by detecting the specimens of head and neck squamous cell carcinoma. Methods: 80 specimens of head and neck squamous cell carcinoma ，including 17 cases of tongue, 13 cases of oral, 14 cases of oropharynx and 36 cases of hypopharynx, were detected by single nucleotide polymorphism chip.In the follow-up period,as the test group 39 patients occurs distant metastasis,as the control group 41 patients did not occur distant metastasis.Results: The results of single nucleotide polymorphism（SNP） showed a distinct peak of frequent genomic gain at 11q13. Using Cox analysis of the array data, we found relative risk of T allele of rs9651738 as the snp probe of SHANK2 gene was 3.61（P=0.003）, relative risk of T allele of rs4980690 as the snp probe of FGF4 gene was 3.63（P=0.003）, relative risk of T allele of rs7929885 as the snp probe of ANO1 gene was 4.35（P=0.008）, relative risk of T allele of rs687660 as the snp probe of PPFIA1 gene was 4.24(P=0.011) , relative risk of T allele of rs6606651 as the snp probe of ORAOV1 gene was 3.04(P=0.013), relative risk of T allele of rs592412 as the snp probe of CCND1 gene was 4.07(P=0.013). Conclusion: These genes at 11q13 can increase the risk of metastasis of head and neck squamous cell carcinoma and possibly become the therapeutic target.These genes include T allele of rs9651738 in SHANK2, T allele of rs4980690 in FGF4 , T allele of rs7929885 in ANO1 , T allele of rs687660 in PPFIA1 , T allele of rs6606651 in ORAOV1 and T allele of rs592412 in CCND1. |
| Key words: head and neck cancer,gene microarray, metastasis |
