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香草酸瞬时受体亚型1基因敲除雌性小鼠痛阈的变化
巩琦,卢占英,余立华,马蓓*
0
(第二军医大学基础部生理学教研室,上海 200433
共同第一作者
*通信作者)
摘要:
目的 探讨TRPV1基因敲除后外周痛觉的改变。方法 采用甩尾热痛仪和弗莱毛测痛法测量TRPV1基因敲除型及野生型雌性小鼠的热和机械痛阈,并进行比较。结果 热刺激后,TRPV1基因敲除型雌性小鼠较野生型雌性小鼠的甩尾潜伏期延长[(3.59±0.65) s vs (2.19±0.24) s,P<0.05],两组间机械痛阈值差异无统计学意义[(1.71±0.57) g vs (2.13±0.81) g,P>0.05]。结论 在生理条件下,TRPV1受体介导热刺激痛,与机械刺激痛无明显相关。
关键词:  TRPV阳离子通道  基因敲除小鼠  痛阈
DOI:10.3724/SP.J.1008.2011.01013
投稿时间:2011-05-27修订日期:2011-07-01
基金项目:
Changes of peripheral pain threshold in transient receptor potential vanilloid 1 knockout female mice
GONG Qi,LU Zhan-ying,YU Li-hua,MA Bei*
(Department of Physiology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China
Co-first authors.
*Corresponding author.)
Abstract:
ObjectiveTo investigate the changes of peripheral pain threshold after knockout of transient receptor potential vanilloid 1(TRPV1) gene. MethodsTail-Flick Analgesia Meter and von-Frey hair were used to determine the peripheral thermal and mechanical thresholds in TRPV1 knockout and wild-type female mice, and the results of the two groups were compared. ResultsThe Tail-Flick latency in TRPV1 knockout mice was significantly prolonged after hot stimulation compared with that in the wild-type group(\[3.59±0.65\] s vs \[2.19±0.24\] s, P<0.05).There was no significant change in responses to von-Frey hair stimuli between the two groups(\[1.71±0.57\] g vs \[2.13±0.81\] g,P>0.05). ConclusionIt is suggested that TRPV1 receptor mediate thermal stimuli response under physiological condition, and has no noticeable influence on mechanical stimuli response.
Key words:  TRPV cation channels  knockout mice  pain threshold