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| 海洋盐单胞菌属微生物的鉴定及生物学活性分析 |
| 赵贵成△,张韬△,高云,卢小玲,龙聪,刘军华,刘小宇*,焦炳华* |
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| (第二军医大学基础部生物化学与分子生物学教研室,上海200433) |
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| 摘要: |
| 目的对36株海洋盐单胞菌进行16S rDNA的序列测定和生物学活性的初步分析。方法用PCR方法扩增36株细菌的16S rDNA序列,将获得的全部序列运用Clustal X1.8软件进行多序列比对并用MEGA4.1软件绘制进化树。利用MTT法测定菌株发酵液的细胞毒活性；采用DPPH和ABTS抗氧化模型检测菌株发酵液的抗氧化活性；采用人白细胞弹性蛋白酶(human leukocyte elastase, HLE)抑制剂筛选模型检测菌株发酵液对HLE的抑制率。结果经与GenBank数据库比对,36株菌均与盐单胞 |
| 关键词: 盐单胞菌科 鉴定 细胞毒素类 抗氧化剂 白细胞弹性蛋白酶 |
| DOI：10.3724/SP.J.1008.2011.0813 |
| 投稿时间：2011-05-31修订日期：2011-06-23 |
| 基金项目:国家高技术研究发展计划(“863”计划, 2011AA09070302). |
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| Identification and bioactivities analysis of Halomonas sp. from seawater |
| ZHAO Gui-cheng△,HANG Tao△,GAO Yun,LU Xiao-ling,LONG Cong,LIU Jun-hua,LIU Xiao-yu*,JIAO Bing-hua* |
| (Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| ObjectiveTo determine the 16S rDNA sequences of 36 strains of Halomonas sp. isolated from the seawater of the East China Sea and to study their bioactivities. MethodsThe 16S rDNA sequences of all isolated strains were amplified by PCR, and the obtained se |
| Key words: Halomonadaceae identification cytotoxins antioxidants leukocyte elastase |
