| 摘要: |
| 目的 探讨大学生血浆多不饱和脂肪酸(polyunsaturated fatty acids,PUFAs)含量与疲劳的关系。方法 血浆样本来自广州市某大学65位健康大学生,多不饱和脂肪酸检测方法 为气相色谱法(GC)。采用程序升温法，初始柱温170℃,经3℃/min升温至210℃并保持6 min,再以10℃/min升温至230℃并保持46 min。检测指标有亚油酸(linoleic acid,LA)、花生四烯酸(arachidonic acid,AA)、α-亚麻酸(α-linolenic acid,α-LNA)、二十碳五烯酸(eicosapentaenoic acid,EPA)和二十二碳六烯酸(docosahexaenoic acid,DHA)。采用酸催化法(H2 SO4 /甲醇)对多不饱和脂肪酸进行甲酯化,内标法(正十七酸)定量测定。研究对象的疲劳测评采用简明心境量表(brief profile of mood states,BPOMS)中的疲劳亚量表。结果 不同性别大学生血浆中AA含量的差异有统计学意义(P<0.01)；不同性别大学生在疲劳得分上的差异无统计学意义(P>0.05)；男生、女生以及全体调查对象α-LNA含量与疲劳得分均呈负相关(r=-0.454 3,r=-0.342 2,r=-0.367 4,P均＜0.05)；同时,按疲劳情绪得分中位数分组,把调查对象划分为高分组(n=33)和低分组(n=32),结果 高、低分组血浆中α-LNA水平、蛋类和坚果类食品的摄入频率差异均有统计学意义(P<0.01或P<0.05)；多不饱和脂肪酸和疲劳(Y)的多重线性回归分析显示,只有α-LNA(X)进入最后的回归模型,回归方程为:Y=10.256 1-0.029 35X(t=2.59,P=0.090 8)。结论 疲劳现象在大学生中普遍存在,疲劳与α-LNA水平呈负相关；大学生应养成科学的饮食习惯,多摄入富含ω-3 多不饱和脂肪酸尤其是α-LNA的食物以预防和缓解疲劳。 |
| 关键词: 多不饱和脂肪酸 疲劳 量表 大学生 |
| DOI：10.3724/SP.J.1008.2012.001099 |
| 投稿时间：2011-12-12修订日期：2012-06-20 |
| 基金项目: |
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| Plasma content of polyunsaturated fatty acids and its relationship with fatigue among college students |
| LIANG Tian1,2,SUN Zhen-xin1,SUN Yuan-ming1,2,YANG Yi-chao1,WU Zhan-bo1,LIU Chun-hong1,2* |
(1. Department of Food Science, College of Food Science,South China
Agricultural University, Guangzhou 510642, Guangdong, China
2. Key Laboratory of Food Safety of Guangdong Province,Guangzhou 510642, Guangdong, China
*Corresponding author.) |
| Abstract: |
| ObjectiveTo explore the relationship between plasma content of polyunsaturated fatty acids (PUFAs) and fatigue among college students. MethodsA total of 65 healthy voluntary subjects were recruited from a university in Guangzhou. Plasma samples were collected and PUFAs levels were determined by gas chromatography (GC). The temperature program ramped from 170℃ to 210℃ at 3℃ per minute for 6 minutes, then from 210℃ to 230℃ at 10℃ per minute for 46 minutes. The examination items included: linoleic acid (LA), arachidonic acid (AA), α-linolenic acid (α-LNA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Acid catalysis method was used for catalyzing methyl esterification of fatty acid, and an internal standard method using heptadecanoic acid was adopted for direct and quick determination of PUFAs in plasma. Fatigue sub-scale of brief profile of mood states (BPOMS) was used for fatigue evaluation. ResultsSignificant gender difference was found in AA levels among the subjects (P<0.01) and no gender difference was found for fatigue score (P>0.05). Levels of α-LNA were negatively correlated with the fatigue score among males, females and all the subjects (r=-0.454 3, r=-0.342 2, and r=-0.367 4, all P<0.05). The subjects were divided into a high-score group (n=33) and a low-score group (n=32) according to fatigue scores. And we found that the α-LNA levels and intake frequencies of eggs and nuts were significantly different between the high-score group and the low-score group( P<0.01 or P<0.05). Multiple linear regression analysis for the factors of fatigue (Y) showed that only α-LNA (X) entered the model after adjusting, with the regression equation being: Y=10.256 1-0.029 35 X (t=2.59, P=0.090 8). ConclusionFatigue is common among college students, and it is negatively correlated with the α-LNA level. Students should develop a scientific diet and take more food rich in ω-3 fatty acid, especially in α-LNA so as to effectively prevent and alleviate fatigue. |
| Key words: polyunsaturated fatty acids fatigue scale college students |
