| 摘要: |
| 目的探讨大鼠非梗死区心肌Dock1蛋白的表达改变。 方法建立SD大鼠实验性心肌梗死(22只)及假手术模型(20只)。分别于术后24 h和12周时处死心肌梗死组和假手术组大鼠(各8只),获取心脏标本,应用免疫印迹法检测非梗死区心肌及假手术组心肌Dock1蛋白的表达。 结果术后24 h时,心肌Dock1蛋白表达在梗死组(0.13±0.03)与假手术组(0.10±0.04)间的差异无统计学意义(P>0.05)；但术后12周时,梗死组(0.17±0.04)较假手术组(0.11±0.05)表达增加,差异有统计学意义(P<0.05); 术后12周时的梗死组心肌Dock1蛋白表达较24 h时的梗死组和假手术组均增加,差异有统计学意义(P<0.05)。结论心肌中存在Dock1蛋白的表达；梗死后12周时非梗死区心肌Dock1蛋白表达增加。 |
| 关键词: Dock1蛋白 鸟苷交换因子 心肌梗死 心肌 |
| DOI：10.3724/SP.J.1008.2012.00540 |
| 投稿时间：2012-02-13修订日期：2012-03-19 |
| 基金项目:重庆市卫生局医学科学技术研究项目(2009-2-290, 04-2-154)，重庆市科委自然科学基金(CSTC 2007BB5276). |
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| Change of Dock1 protein expression in non-infarcted myocardium in rats |
| LIU Xiao-lan,ZHAO Wen-ju,LI Gang﹡,WANG Zhi-hua,WANG Li-ping |
(Division of Cardiology, Department of Geriatrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
*Corresponding author.) |
| Abstract: |
| ObjectiveTo investigate the change of Dock1 protein expression in non-infarcted myocardium in rats. MethodsExperimental myocardial infarction models were established in 22 SD rats (MI group) and the other 20 rats received sham-operation (Sham group). Eight rats were sacrificed 24 h after operation in each group and another 8 were sacrificed at 12 weeks after operation; the cardiac specimens were collected. Dock1 protein expression was measured by Western blotting analysis in the non-infarcted myocardium of MI group and sham group. ResultsDock1 protein expression was not significantly different in the non-infarcted myocardium between MI group (0.13±0.03) and Sham group (0.10±0.04) at 24 h after operation (P>0.05); 12 weeks after operation, the expression in non-infarcted myocardium of MI group (0.17±0.04) was significantly higher than that of Sham group (0.11±0.05, P<0.05). Dock1 protein expression in non-infarcted myocardium was significantly increased in MI group at 12 weeks after operation compared with those in MI and Sham groups at 24 h after operation (P<0.05). ConclusionThere is Dock1 protein expression in rat’s myocardium, and the expression is significantly increased in the non-infarcted myocardium at 12 weeks after infarction. |
| Key words: Dock1 protein guanine nucleotide exchange factor myocardial infarction myocardium |
