| 摘要: |
| 目的 对比分析高糖高脂饲料诱导的肥胖大鼠和相同饲料联合小剂量链脲佐菌素(STZ)制备的糖尿病大鼠糖脂代谢特点。方法 雄性SD大鼠随机分为正常组(NC组)和高脂组(HF组),分别给予正常饲料或高糖高脂饲料喂养8周,8周后在HF组中选取肥胖大鼠再次随机分为两组,一组为糖尿病组(DM组),注射小剂量STZ 30 mg/kg；另一组为肥胖组(OB组)。各组继续原饲料喂养并观察4周。测定大鼠体质量、血糖、三酰甘油、总胆固醇、空腹胰岛素、胰岛素敏感性,并进行腹腔内糖耐量试验和胰岛素释放试验。结果 高脂联合注射STZ后,DM组逐渐出现了多饮、多食、多尿和体质量减轻的特点。第12周末,DM组空腹血糖高于OB组(P<0.01),胰岛素敏感性低于OB组(P<0.01),葡萄糖耐量、胰岛素分泌功能受损程度大于OB组,血清总胆固醇、三酰甘油水平高于OB组(P<0.01)。结论 持续高糖高脂饮食能够诱导大鼠出现糖调节受损、胰岛分泌功能减退以及脂代谢紊乱。高脂联合注射STZ后,糖尿病大鼠的上述受损幅度明显加大。 |
| 关键词: 肥胖症 胰岛素抵抗 2型糖尿病 |
| DOI：10.3724/SP.J.1008.2012.00343 |
| 投稿时间：2012-03-06修订日期：2012-03-06 |
| 基金项目: |
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| High fat diet-induced obese rats and diabetic rats: a comparative study of glucose and lipid metabolism |
| LI Juan1,ZOU Da-jin2 *,FENG Zheng-kang2 |
(1. Department of Nutrition, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
2. Department of Endocrinology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
*Corresponding author.) |
| Abstract: |
| Objective To compare the glucose and lipid metabolism between high sucrose plus high fat diet-induced obese rats and diabetic rats prepared with the same feed combined with low-dose streptozotocin. Methods Six to eight weeks old SD rats were randomly divided into normal control group and high fat group, fed with normal diet and high sucrose plus high fat diet for 8 weeks, respectively. Eight weeks later HF group was further randomized into two groups: one is diabetic mellitus group(DM group) injected with low dose streptozotocin (30 mg/kg), the other is obese group(OB group). The animals were maintained on their diet and were observed for 4 weeks. The body weight, blood glucose, triglyceride, total cholesterol, fasting insulin, insulin sensitivity were examined. Intraperitoneal glucose tolerance test (IPGTT) and insulin release test were also performed. Results After treatment with high fat diet plus STZ injection, DM group gradually developed symptoms of excessive food & water consumption, polyuria and weight loss. At the end of the 12th week, the fasting blood glucose of DM group was significantly higher than that of the OB group (P<0.01 ) and the insulin sensitivity was significantly lower than that of the OB group (P<0.01 ). The rats of OB group presented impaired glucose tolerance and impaired insulin secretion, and DM group had more severely impaired glucose tolerance and impaired insulin secretion; and the serum total cholesterol, triglyceride levels in DM group were significantly higher than those in the OB group (P<0.01 ). Conclusion Continuous high sucrose-high fat diet can induce impaired glucose regulation, insulin secretion dysfunction and lipid metabolism disorders; high fat combined with STZ injection can further aggravate these disorders. |
| Key words: obesity insulin resistance type 2 diabetes |
