| 摘要: |
| 目的探讨蛋白激酶A(PKA)和蛋白激酶C(PKC)对分离培养的背根神经节(DRG)神经元ATP受体P2X3功能的调节作用。方法分离培养大鼠DRG神经元,给予外源性ATP诱导出瞬时型内向电流,通过全细胞膜片钳记录的方法观察P2X3和P2X2/3受体特异性阻断剂TNP-ATP对这一电流的影响,在此基础上观察PKA和PKC激动剂对ATP诱导的瞬时型内向电流的调节作用。结果在分离培养的DRG神经元上,ATP诱导的瞬时型电流可以被TNP-ATP抑制,其抑制效应呈剂量依赖性,半数有效剂量(IC50)为(21.7±7.6) nmol/L。PKA激动剂forskolin(1 μmol/L)和PKC激动剂PMA(1 μmol/L)均可以快速、可逆地抑制ATP诱导的瞬时型电流。结论在大鼠分离培养的DRG神经元,PKA和PKC可能通过磷酸化调节抑制P2X3受体的功能,从而抑制ATP诱导的瞬时型内向电流,提示蛋白激酶对P2X3受体的调节作用可能参与痛觉的形成。 |
| 关键词: 背根神经节 P2X3受体 蛋白激酶A 蛋白激酶C 腺苷三磷酸 |
| DOI：10.3724/SP.J.1008.2012.00813 |
| 投稿时间：2012-04-28修订日期：2012-06-11 |
| 基金项目:上海市科委基础重点项目(08JC1405400). |
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| Regulatory effect of protein kinase A and protein kinase C on P2X3-mediated inward current in rat dorsal root ganglion neurons |
| LI Na1,2△,LU Yi1△,FAN Juan1△,DENG Xiao-ming2*,MA Bei1* |
(1. Department of Physiology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China
2. Department of Anesthesiology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
△Co-first authors.
*Corresponding authors.) |
| Abstract: |
| ObjectiveTo investigate the regulatory effect of protein kinase A (PKA) and protein kinase C (PKC) on the function of ATP receptor P2X3 in cultured dorsal root ganglion (DRG) neurons. MethodsRat DRG neurons were collected, cultured and subjected to exogenous ATP treatment to induce transient inward current. Whole-cell patch clamp recording was employed to observe the effect of TNP-ATP (P2X3 and P2X2/3 receptor antagonist) on the induced current. Meanwhile, the regulatory effects of PKA and PKC agonist on ATP-induced transient inward current were also observed. ResultsTNP-ATP inhibited ATP-induced transient current in cultured DRG neurons in a dose-dependent manner, with the IC50 value being (21.7±7.6) nmol/L. PKA agonist forskolin (1 μmol/L) and PKC agonist PMA (1 μmol/L) inhibited ATP-induced transient current rapidly and reversibly. ConclusionPKA and PKC can inhibit ATP-induced transient inward current in cultured DRG neurons, probably through inhibiting the function of P2X3 receptors. It indicates that P2X3 receptor regulation by protein kinases may participate in the development of pain. |
| Key words: dorsal root ganglion P2X3 receptor protein kinase A protein kinase C adenosine triphosphate |
