| 摘要: |
| 目的探讨ZEB-1在肝细胞癌(HCC)组织中的表达及意义。方法采用免疫组织化学SP法和蛋白质印迹分析法检测ZEB-1在62例HCC组织、48例相应癌旁肝硬化组织和10例正常肝组织中的表达,并分析其与HCC临床病理特征的关系。结果免疫组化结果显示ZEB-1在HCC组织及癌旁肝硬化组织中呈阳性表达,阳性率分别为93.5%(58/62)和83.3(40/48)。蛋白质印迹分析显示ZEB-1蛋白在HCC组织中的表达高于相应癌旁肝硬化组织和正常肝组织 (P<0.05)。ZEB-1在HCC组织中的表达与临床分期、肿瘤分化程度、有无发生转移、有无门脉癌栓及有无术后复发等因素相关(P<0.05),与肿瘤直径、肿瘤数目、血清AFP水平等因素无关(P>0.05)。结论ZEB-1的高表达与肝癌的发生、发展密切相关,并且有可能参与HCC细胞的上皮间质转化而促进肝癌的侵袭和转移。 |
| 关键词: 肝肿瘤 肝细胞癌 ZEB-1 免疫组织化学 |
| DOI：10.3724/SP.J.1008.2012.00842 |
| 投稿时间：2012-05-24修订日期：2012-07-11 |
| 基金项目:国家科技重大专项 (2008ZX10002-025). |
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| Expression of ZEB-1 in hepatocellular carcinoma tissues and its significance |
| YANG Xiao-yu1,FAN Fei2,FU Xiao-hui1,LI Ai-jun1* |
(1. Department of Special Treatment Ⅱ, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China
2. Department of Biliary Tract Surgery Ⅱ, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China
*Corresponding author.) |
| Abstract: |
| ObjectiveTo investigate ZEB-1 protein expression in hepatocellular carcinoma (HCC) tissues and the relevant significance. MethodsImmunohistochemical method and Western blotting analysis were used to detect the ZEB-1 protein expression in 62 HCC tissues,48 paracancerous cirrhotic tissues and 10 normal liver tissues. The relationship between ZEB-1 protein expression and clinicopathological features of HCC was evaluated. ResultsImmunohistochemical staining showed that the positive rates of ZEB-1 protein expression in HCC tissues and paracancerous cirrhotic tissues were 93.5% (58/62) and 83.3 (40/48), respectively. Western blotting analysis showed that ZEB-1 protein expression in HCC tissues was significantly higher than those in the paracancerous cirrhotic tissues and normal liver tissues (P<0.05). We also found that ZEB-1 expression was significantly correlated with the clinical stage, tumor differentiation degree, metastasis, portal vein tumor thrombus and postoperative recurrence (P<0.05), and was not correlated with the tumor diameter, number of tumors, or serum AFP levels (P>0.05). ConclusionHigh expression of ZEB-1 is correlated with the development and progression of HCC. ZEB-1 might also participate in the epithelial-mesenchymal transition of HCC cells, subsequently contributing to the invasion and metastasis. |
| Key words: liver neoplasms hepatocellular carcinoma paracancerous tissue ZEB-1 immunohistochemistry |
