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| 中枢一氧化氮对心血管作用的多样性研究 |
| 章汝文1,2△,沈镀1,2△,孙巍巍3△,王杨凯1,白洁2,袁文俊1,2,王伟忠1* |
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(1. 第二军医大学基础部生理学教研室,上海 200433;
2. 宁夏医科大学基础医院院生理系,银川 750004;
3. 第二军医大学基础部计算机教研室,上海 200433
△共同第一作者
*通信作者) |
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| 摘要: |
| 目的 观察一氧化氮(nitric oxide, NO)在心血管中枢孤束核(NTS)和头端延髓腹外侧区(RVLM)内的心血管作用特点,从而明确其在介导中枢心血管功能调控中的意义。方法 采用微量注射的方法观察麻醉SD大鼠NTS和RVLM增加或降低NO后动物血压、心率和肾交感神经活动的变化。结果 在麻醉SD大鼠模型上,在心血管反射传入的中继站NTS内微量注射NO前体L-精氨酸(L-Arg, 2 nmol/50 nl)能降低基础血压、心率和肾交感神经活动(P<0.05),而注射NO合酶(NOS)抑制剂NG-硝基-L-精氨酸甲酯(L-NAME, 10 nmol/50 nl)能增加心血管功能(P<0.05)。在交感输出的关键区域RVLM注射L-Arg (2 nmol/100 nl)能增加血压、心率和交感神经活动(P<0.05),而给予L-NAME (10 nmol/100 nl)则抑制心血管功能(P<0.05)。结论 NO在不同的心血管中枢中具有不同的作用,提示其多样性效应对维持基础心血管功能具有特定的意义。 |
| 关键词: 一氧化氮 孤束核 头端延髓腹外侧区 血压 |
| DOI：10.3724/SP.J.1008.2012.00718 |
| 投稿时间：2012-04-19修订日期：2012-05-30 |
| 基金项目:国家自然科学基金(30971061,31100831), 第二军医大学青年启动基金(2010QN03). |
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| Multi-effect of central nitric oxide on cardiovascular activity |
| ZHANG Ru-wen1,2△, SHEN Du1,2△, SUN Wei-wei3△, WANG Yang-kai1, BAI Jie2, YUAN Wen-jun1,2, WANG Wei-zhong1* |
(1. Department of Physiology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China;
2. Department of Physiology, Basic Medical School, Ningxia Medical University, Yinchuan 750004, Ningxia, China;
3. Department of Computer, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China
△Co-first authors.
*Corresponding author.) |
| Abstract: |
| ObjectiveTo observe the effect of nitric oxide(NO）on cardiovascular activities in the nucleus tractus solitarii (NTS) and the rostral ventrolateral medulla (RVLM), so as to understand the role of central NO in cardiovascular regulation. MethodsThe changes of blood pressure (BP), heart rate (HR), and renal sympathetic nerve activity (RSNA) were observed following treatments with increased or decreased NO in the NTS and RVLM of anaesthetized SD rats.ResultsMicroinjection of the NO precusor L-arginine (L-Arg, 2 nmol in 50 nl) into the NTS, a key relay of cardiovascular reflex transmission, produced significant decreases in BP, HR, and RSNA (P<0.05), and injection of NO synthase inhibitor NG-nitro-L-arginine methyl ester(L-NAME,10 nmol in 50 nl) into the NTS produced significant increases in these cardiovascular parameters (P<0.05). However, injection of L-Arg (2 nmol in 100 nl) into the RVLM, a key region controlling sympathetic outflow, significantly increased basal BP, HR, and RSNA (P<0.05), and injection of L-NAME (10 nmol in 100 nl) into the RVLM showed a cardiovascular inhibition effect (P<0.05).ConclusionNO exhibits different effects in different cardiovascular centers, suggesting that it plays a special role in maintaining the basal cardiovascular activity. |
| Key words: nitric oxide nucleus tractus solitarii rostral ventrolateral medulla blood pressure |
