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| 透明质酸/聚酰胺-胺三元纳米基因复合物的构建及其体外评价 |
| 王晓宇1,2,孙李平2,朱全刚1,张玮1,3,张敏1,张丽娟1,戴子渊1,高申1* |
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(1. 第二军医大学长海医院药学部,上海 200433
2. 成都军区司令部直属工作部,成都 610000
3. 解放军522医院药材科,洛阳 471003
*通信作者) |
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| 摘要: |
| 目的在聚酰胺-胺(polyamidoamine,PAMAM)树状大分子与DNA形成复合物的基础上添加透明质酸(hyaluronic acid,HA)形成三元纳米复合物,研究其基因递送性能的改变。方法配制不同电荷比的三元纳米复合物,测定其粒径和电位;选择MCF-7和MDA-MB-231细胞进行转染效率的测定，选择B16和MCF-7细胞进行细胞毒性的测定。结果和结论形成的三元纳米复合物大小均一,结构稳定。与单纯的PAMAM载体相比,增加HA提高了转染效率,降低了细胞毒性,适合用于基因的递送和转染。 |
| 关键词: 聚酰胺-胺 透明质酸 三元纳米复合物 转染效率 |
| DOI：10.3724/SP.J.1008.2012.001288 |
| 投稿时间：2012-06-13修订日期：2012-08-27 |
| 基金项目:国家自然科学基金 (81172514, 81101658). |
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| Construction and in vitro evaluation of hyaluronic acid/polyamidoamine ternary nano-gene polyplex |
| WANG Xiao-yu1,2,SUN Li-ping2,ZHU Quan-gang1,ZHANG Wei1,3,ZHANG Min1,ZHANG Li-juan1,DAI Zi-yuan1,GAO Shen1* |
(1. Department of Pharmacy, Changhai Hosptial, Second Military Medical University, Shanghai 200433, China
2. Subordinate Service Department, Headquarters of PLA Chengdu Military Area Command, Chengdu 610000, Sichuan, China
3. Department of Pharmacy, No. 522 Hospital of PLA, Luoyang 471003, Henan, China
*Corresponding author.) |
| Abstract: |
| ObjectiveTo prepare the ternary nano-gene polyplex by adding hyaluronic acid (HA) into DNA/polyamidoamine (PAMAM) binary nano-gene polyplex, and to probe into its gene transfection ability. MethodsDNA and HA were mixed with PAMAM dendrimer in different charge ratios to form ternary nano-gene polyplex, and the size and zeta potential of the polyplex were measured. MCF-7 and MDA-MB-231 cells were used for transfection efficiency test; B16 and MCF-7 cells were used for cell viability assay. Results and conclusionThe prepared ternary nano-gene polyplex has even size and stable structure. HA can enhance the gene transfection efficiency and reduce the cell toxicity compared with pure PAMAM, making it suitable for gene delivery and transfection. |
| Key words: polyamidoamine hyaluronic acid ternary nano-gene polyplex transfection efficiency |
