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高效液相色谱法测定肝力保胶囊中苦参碱及槐定碱的含量
王慧
0
(第二军医大学东方肝胆外科医院)
摘要:
[摘要] 目的 建立高效液相色谱法测定肝力保胶囊中苦参碱及槐定碱含量。方法 使用Agilent NH2 (4.6 mm×250 mm, 5μm),流动相:1%磷酸:无水乙醇:乙腈=10:22:68,流速0.8 ml/min,柱温25℃,检测波长210nm,测定15批肝力保胶囊中苦参碱及槐定碱含量。结果 苦参碱在5.21-156.3 μg/ml (r=1),槐定碱在5.29-158.7 μg/ml(r=0.9999)浓度范围内呈良好线性关系,苦参碱回收率为98.73% (RSD=3.67 %),槐定碱回收率为100.10%(RSD=2.24%)。15批肝力保胶囊中苦参碱含量在9.04-13.4 1 mg/粒之间,槐定碱含量在6.48-15.62 mg/粒之间。结论 该方法简便、准确,重复性好,可用于肝力保胶囊的质量控制。
关键词:  高效液相色谱法  苦参碱  槐定碱  肝力保胶囊  含量测定
DOI:10.3724/SP.J.1008.2012.001267
投稿时间:2012-06-25修订日期:2012-10-25
基金项目:
Determination of matrine and sophoridine contents in ganlibao capsule by High Performance Liquid Chromatography
wanghui
()
Abstract:
Objective To establish a high performance liquid chromatography approach for determining matrine and sophoridine contents in Ganlibao capsule. MethodsAgilent NH2 (4.6 mm×250 mm, 5 μm) column was used with 1% phosphoric acidethanolacetonitrile (V/V/V=102268) as the mobile phase. The flow rate was 0.8 ml/min, and the temperature was at 25℃.The detection wavelength was set at 210 nm. Results The calibration curve of matrine showed a good linearity in the range of 5.21-156.3 μg/ml (r=1) and that of sophoridine in the range of 5.29-158.7 μg/ml (r=0.999 9). The equations of linear regression of matrine and sophoridine were Y=9.586 1X+1.003 8 and Y=7.604 3X-5.920 1, respectively. The recovery was 98.73% (RSD=3.67%) for matrine and 100.10% (RSD=2.24%) for sophoridine. The matrine content of 15 batches of Ganlibao capsule was (30.13±0.19)-(44.70±0.17) mg/g and the sophoridine content was (21.60±0.25)-(52.07±0.11) mg/g. Conclusion The present method is simple, accurate, and reproducible, and it can be used for the quality control of Ganlibao capsule.
Key words:  HPLC  matrine  sophoridine  ganlibao capsule  determination