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3种全自动血型仪在交叉匹配试验中的应用
陈波,葛春红,林艳,刘银,周晔,唐晓峰*
0
(第二军医大学长征医院输血科,上海 200003
*通信作者)
摘要:
目的 探讨WADianaR○(戴安娜)、Techno TwinStation、ORTHO AutoVue Innova/Ultra 3种全自动血型/配血系统分析仪在临床交叉匹配试验中应用的效果,以确保临床输血的安全。方法 用3种全自动血型/配血系统分析仪对临床备血的8 073例受血者与上海市血液中心提供的供血者标本进行交叉匹配试验操作,将血液交叉匹配试验阳性标本用抗人球蛋白试管法的交叉匹配试验进行确认。结果 3种全自动血型/配血系统分析仪共检出589例阳性的备血标本。经抗人球蛋白试管法的交叉匹配试验确认,WADianaR○全自动血型/配血系统分析仪未出现假阳性结果,Techno TwinStation和ORTHO AutoVue Innova/Ultra的分析仪分别出现了6例和19例假阳性结果。结论 3种全自动血型/配血系统分析仪在交叉匹配试验中都能准确判断阳性的标本,以WADianaR○系统分析仪的效果更为突出。
关键词:  血型鉴定和交叉配血  全自动血型/配血系统分析仪  微柱法  免疫血型学试验
DOI:10.3724/SP.J.1008.2013.0092
投稿时间:2012-07-31修订日期:2012-11-07
基金项目:上海市公共卫生重点学科建设计划项目 (12GWZX0202).
Application of three automatic blood group testing instruments in cross matching test
CHEN Bo,GE Chun-hong,LIN Yan,LIU Yin,ZHOU Ye,TANG Xiao-feng*
(Department of Blood Transfusion, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
*Corresponding author.)
Abstract:
Objective To apply three automatic blood group testing instruments, WADianaR○ (Diana), Techno TwinStation and ORTHO AutoVue Innova/Ultra, in clinical cross match test and to assess their effect, so as to ensure safe clinical blood transfusion. Methods The three instruments were used for cross matching test of 8 073 blood recipients and blood specimens of Shanghai Blood Center. The incompatible positive samples were reexamined by experiment cross matching antiglobulin test tube method. Results The 3 instruments detected 589 positive blood samples. There was no false positive result in the WADianaR○(Diana) system; Techno TwinStation and ORTHO AutoVue Innova/Ultra analyzer had 6 and 19 false positive results, respectively. Conclusion All the three instruments can accurately detect the positive samples in clinical cross match test, and WADianaR○ system has a more prominent performance.
Key words:  blood grouping and crossmatching  automatic blood analyzer/blood matching system  micro column chromatography  blood group immune test