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| 脾切除对门脉高压大鼠种植性肝肿瘤的影响 |
| 范鲁峰1,杨宁1,汪慧2,邵卓3,赵文超1,陈本栋1,付雍1,杨广顺1* |
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(1.第二军医大学东方肝胆外科医院肝外五科,上海 200438
2.第二军医大学东方肝胆外科医院肝胆内一科,上海 200438
3.第二军医大学长海医院普通外科,上海 200433
*通信作者) |
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| 摘要: |
| 目的通过建立大鼠门脉高压背景下肝肿瘤模型,观察病理性脾脏的切除对肝肿瘤发生发展的影响。方法按照四氯化碳复合、梯段用药法制备大鼠肝硬化门脉高压模型,后将Walker-256肿瘤块种植在大鼠肝脏左外叶上,随机分组的A组大鼠在种植瘤块同时切除脾脏,B组大鼠保留脾脏。10 d后分别测定两组大鼠的T细胞亚群CD4、CD8及CD4/CD8；切除大鼠肝脏左外叶,测量种植肿瘤块最大直径,并用免疫组织化学染色方法检测肿瘤组织Ki-67的表达。结果门脉高压模型建成后,大鼠的血常规以及肝功能指标出现明显异常。A组大鼠的CD4值和CD4/CD8比值均高于B组大鼠(0.36±0.01 vs 0.35±0.02,1.33±0.08 vs 1.24±0.05；P<0.05或P<0.01),而CD8值则低于B组大鼠(0.27±0.01 vs 0.29±0.02,P<0.01)。A组大鼠的肝脏肿瘤直径与B组大鼠比较差异无统计学意义。A组大鼠肿瘤组织的Ki-67阳性率较B组降低,差异具有统计学意义(P<0.05)。结论门脉高压状态下病理性脾脏的切除既有利于提高机体抗肿瘤的免疫力,又可以在一定程度上减弱肝肿瘤的侵袭能力。 |
| 关键词: 脾切除术 门静脉高压 肝肿瘤 免疫 |
| DOI：10.3724/SP.J.1008.2012.000 |
| 投稿时间：2012-10-18修订日期：2012-11-22 |
| 基金项目:国家传染病重大科技专项课题（2012ZX10002-017）. |
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| Effect of splenectomy on implanted hepatic tumor rat model with portal hypertension |
| FAN Lu-feng1,YANG Ning1,WANG Hui2,SHAO Zhuo3,ZHAO Wen-chao1,CHEN Ben-dong1,FU Yong1,YANG Guang-shun1* |
(1. Fifth Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China
2. Department of Hepatobiliary Disease Ⅰ, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China
3. Department of General Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
*Corresponding author.) |
| Abstract: |
| ObjectiveTo evaluate the effect of splenectomy on development and progression of implanted hepatic tumors by establishing implanted hepatic tumor rat model with portal hypertension. MethodsRat cirrhosis with portal hypertension models were built by subcutaneous injection of carbon tetrachloride, and then the rats were randomly divided into two groups. Group A was subjected to splenectomy and implantation of Walker-256 tumor tissue on the left lobe of liver, and group B was only subjected to implantation of tumor tissue. T-lymphocyte subsets CD4, CD8 and CD4/CD8 of two groups were examined 10 days after implantation of tumor tissue; the left lobe of liver was resected and the maximal tumor size was measured; expression of Ki-67 was detected by immunohistochemical method in the tumor tissues. ResultsThe results of blood routine test and liver function test were obviously abnormal in rat model of portal hypertension. The levels of CD4 and CD4/CD8 in group A were higher than those in group B (0.36±0.01 vs 0.35±0.02,1.33±0.08 vs 1.24±0.05; P<0.05 or P<0.01), and the level of CD8 in group A was lower than that of CD8 in group B (0.27±0.01 vs 0.29±0.02, P<0.01). There was no significant difference in the diameter of hepatic tumor between the two groups. Ki-67-positive rate of tumor in group A was lower than that in group B (P<0.05). ConclusionSplenectomy in portal hypertension rats can not only help to improve the immunity against hepatic tumor, but also decrease the invasive ability of the hepatic tumor to some extent. |
| Key words: splenectomy portal hypertension liver neoplasms immunity |
