| 摘要: |
| 目的 优化胸腹水液基细胞学剩余标本制作细胞块的程序,并探讨其在病理诊断中的应用价值。方法 150例胸腹水薄层液基细胞学技术(thinprep cytologic test, TCT)检测剩余标本,根据细胞学诊断结果分为3组,每组50例,分别采用直接离心法、蛋清液作为支架法和细胞块试剂盒法处理后制作石蜡细胞块,对比分析3种不同方法制成的细胞切片上的恶性细胞检出率、细胞分布状况和形态特征,并初步比较细胞块和组织块免疫化学染色效果。结果 150例胸腹水TCT检测出的恶性细胞率为31.3%(47/150),其剩余标本经细胞块法检测出的总恶性细胞检出率为40.7%(61/150),其中直接离心法、蛋清液作为支架法和细胞块试剂盒法的检出率分别为26.0%(13/50)、46.0%(23/50)和50.0%(25/50) 。用蛋清液作为支架及细胞块试剂盒两种方法制作的细胞块,恶性细胞检出率高于直接离心法(P<0.05),细胞聚集度和细胞分布也优于直接离心法,细胞块和组织块免疫化学染色效果相似。结论 用蛋清液作为支架及细胞块试剂盒两种方法制作的细胞块可提高胸腹水液基细胞学剩余标本的恶性细胞检出率,并可用于免疫细胞化学检测。 |
| 关键词: 胸腔积液；腹水 液基细胞学 细胞块 石蜡包埋 |
| DOI：10.3724/SP.J.1008.2013.00160 |
| 投稿时间：2012-10-30修订日期：2013-01-03 |
| 基金项目:上海中医药大学“085”一流学科建设科技创新支撑计划(085ZY1220). |
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| Preparation of cell blocks using residual pleural and peritoneal effusion: a comparison of three methods |
| WANG Shou-mei,YANG Qiu-hong,WEI Jing-jing,WANG Yu-jia,ZHANG Shu-hui* |
(Department of Pathology,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
*Corresponding author.) |
| Abstract: |
| Objective To optimize the process of making cell blocks by residual pleural and peritoneal effusions, and to explore its value in pathological diagnosis. Methods Totally 150 residual pleural and peritoneal effusion specimens of thinprep cytologic test (TCT) were evenly divided into 3 groups according to cytopathological diagnosis. Specimens in the three groups were processed by direct centrifugal method, egg white as the bracket method and cell block test method. The detection rate of malignant cells, distribution status and morphological features of cells on the cellular sections were compared between the three different methods, and the immunohistochemical staining results were compared between the cell block and tissue block. Results The TCT yielded a detection rate of malignant cells of 31.3% (47/150), and examination of the cell block in this study yielded a detection rate of 40.7% (61/150), with the rates of direct centrifugal method, egg white as the bracket method and cell block test method being 26.0%(13/50), 46.0%(23/50) and 50.0%(25/50), respectively. The detection rates of malignant cells in egg white as the bracket method and cell block test method groups were significantly higher than that in the direct centrifugation method (P<0.05); in addition, the former two groups also had better cell aggregation and distribution. The immunohistochemical staining results of cell blocks were also similar to those of tissue blocks. Conclusion The cell blocks processed by egg white as the bracket method and cell block test method can improve the detection rate of malignant cells in residual pleural and peritoneal effusion, and the blocks can be used for immunocytochemistry staining. |
| Key words: pleural effusion ascites thinprep cytologic test cell block paraffin-embedded |
