| 摘要: |
| 目的 探讨褪黑素受体激动剂Neu-P11对胰岛素抵抗3T3-L1脂肪细胞IRS-1和GLUT-4表达的影响。方法 用高糖高胰岛素作用3T3-L1脂肪细胞24 h,建立胰岛素抵抗细胞模型。分别采用褪黑素、Neu-P11、褪黑素+luzindole(褪黑素受体拮抗剂)和Neu-P11+luzindole处理细胞,并以未加药细胞作为对照。用葡萄糖氧化酶法检测培养液中的糖含量并计算糖消耗量,用蛋白质印迹分析检测细胞内IRS-1、GLUT-4蛋白表达变化。结果 胰岛素抵抗细胞用褪黑素和Neu-P11处理后,糖消耗量较未加药对照组升高(P<0.05),GLUT-4、IRS-1的蛋白表达也增高(P<0.05)；褪黑素和Neu-P11分别与luzindole联合作用于细胞后,细胞的上述蛋白表达较单用褪黑素组和单用Neu-P11组降低(P<0.05),而与未加药对照组相比差异无统计学意义。结论 褪黑素受体激动剂Neu-P11可提高胰岛素抵抗脂肪细胞的胰岛素敏感性,增加糖消耗量,这种作用可能与上调IRS-1、GLUT-4蛋白的表达有关。 |
| 关键词: Neu-P11 褪黑素 脂细胞 胰岛素抵抗 |
| DOI：10.3724/SP.J.1008.2013.00561 |
| 投稿时间：2012-11-18修订日期：2013-02-04 |
| 基金项目:湖南省教育厅科学研究项目(12C1196),怀化医学高等专科学校科学研究项目(2011K03). |
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| Effect of melatonin receptor agonist Neu-P11 on expression of IRS-1 and GLUT-4 in insulin-resistant adipocytes |
| LI Xiu-ping1,YIN Wei-dong2*,HU Xiao-bo2,SHE Mei-hua2,WANG Ping-ping2,ZHANG Su-jun2,HUANG Liang2 |
(1. Department of Laboratory Medicine, Huaihua Medical College, Huaihua 418000, Hunan, China
2. Institute of Cardiovascular Disease, School of Medicine, University of South China, Hengyang 412000, Hunan, China
*Corresponding author.) |
| Abstract: |
| Objective To explore the influence of melatonin receptor agonist Neu-P11 on the expression of IRS-1 and GLUT-4 in insulin-resistant 3T3-L1 adipocytes. Methods Insulin resistant 3T3-L1 adipocytes were induced with high glucose/high insulin for 24 hours, and then they were divided into 4 treatment groups: melatonin, Neu-P11, melatonin+luzindole and Neu-P11+luzindole. And non-treated insulin-resistant 3T3-L1 adipocytes were taken as control. Glucose consumption was detected by enzymatic method. IRS-1 and GLUT-4 protein expressions were detected by Western blotting analysis. Results After the insulin-resistant adipocytes were treated with melatonin and Neu-P11, the glucose consumption and the expressions of IRS-1, GLUT-4 proteins were significantly increased compared with the non-treated control group (P<0.05). The expressions of IRS-1, GLUT-4 proteins in melatonin+luzindole(melatonin receptor antagonist) and Neu-P11+luzindole groups were significantly decreased compared with melatonin alone or Neu-P11 alone groups (P<0.05), and were similar to those in the non-treated control group. Conclusion Melatonin receptor agonist Neu-P11 can increase glucose consumption, insulin sensitivity in insulin resistant-adipocytes, which might be associated with the up-regulation of IRS-1 and GLUT-4 protein expression. |
| Key words: Neu-P11 melatonin high glucose/high insulin adipocytes insulin resistance |
