| 摘要: |
| 目的 对中国南海黑乳海参共附生白色侧齿霉菌Engyodontium album的次生代谢产物进行研究。方法 运用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、高效液相色谱等分离手段对白色侧齿霉菌Engyodontium album的乙酸乙酯提取物进行分离纯化, 根据现代波谱技术结合文献报道进行结构鉴定。结果 共分离得到5个化合物, 分别鉴定为:(22E,24R)-5α,8α-过氧化麦角甾-6,22-二烯-3β-醇(1)、(22E,24R)-麦角甾-5,7,22-三烯-3β-醇(2)、(22E,24R)-麦角甾-7,22-二烯-3β,5α,6β-三醇(3)、3β,5α-二羟基-(22E,24R)-麦角甾-7,22-二烯-6-酮(4)、3β,5α-二羟基-6β-甲氧基-麦角甾-7,22-二烯(5)。结论 本研究是对黑乳海参共附生白色侧齿霉菌次生代谢产物的首次报道, 这5个化合物均为首次从白色侧齿霉菌Engyodontium album中分离得到。 |
| 关键词: 海参纲 黑乳海参 白色侧齿霉菌 甾类 |
| DOI：10.3724/SP.J.1008.2013.00310 |
| 投稿时间：2013-01-29修订日期：2013-02-20 |
| 基金项目:国家自然科学基金(30873200,41176125), 上海浦江人才计划(PJ2008). |
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| Steroids from fungus Engyodontium album associated with the South China Sea cucumber Holothuria nobilis Selenka |
| GONG Jun,TANG Hua,LIU Bao-shu,ZHOU Wei,SUN Peng,LI Ling*,ZHANG Wen* |
(Research Center for Marine Drugs, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
*Corresponding authors.) |
| Abstract: |
| Objective To investigate the secondary metabolites of fungus Engyodontium album (E.album) associated with the South China Sea cucumber Holothuria nobilis (H. nobilis) Selenka. Methods The EtOAc extract of E.album was purified by repeated column chromatography on silica gel, Sephadex LH-20, and high-performance liquid chromatography (HPLC). Structures of the obtained compounds were determined by spectroscopic analysis and comparison with previously reported data. Results Five compounds were isolated and their structures were determined as: (22E,24R)-5α,8α-epidioxy-ergosta-6,22-dien-3β-ol (1), (22E,24R)-ergosta-5,7,22-trien-3β-ol (2), (22E,24R)-ergosta-7,22-dien-3β,5α,6β-triol (3), 3β,5α-dihydroxy-(22E,24R)-ergosta-7,22-dien-6-one (4), 3β,5α-dihydroxy-6β-methoxy-ergosta-7,22-dien (5). Conclusion This is the first report on the secondary metabolites of E. album associated with the sea cucumber H. nobilis Selenka, and the five compounds have been reported for the first time from the fungus E. album. |
| Key words: sea cucumbers Holothuria nobilis Selenka Engyodontium album steroids |
