| 摘要: |
| 目的 探讨化疗对噬菌体展示肽法检测大肠癌自身抗体效果的影响。 方法 选取前期研究筛选出的5个（95号，149号，174号，396号，1009号）能高效区分大肠癌患者血清和健康对照者血清的大肠癌相关噬菌体克隆作为检测噬菌体，采用酶联免疫吸附试验（ELISA）检测该5个检测噬菌体克隆与20例化疗大肠癌患者、40例非化疗大肠癌患者及40例健康对照者血清的反应性。 结果 95号检测噬菌体与大肠癌化疗患者血清反应性低于与非化疗大肠癌患者血清反应性（P<0.05），该噬菌体在健康对照组及化疗大肠癌患者组的血清反应性差异无统计学意义（P=0.074）。149号、174号、396号、1009号噬菌体在健康对照组及化疗大肠癌患者组的血清反应性差异有统计学意义（P均<0.01）。 结论 化疗对149号、174号、396号、1009号噬菌体的检测效果影响不明显，而95号噬菌体对肿瘤自身抗体的检测能力受化疗影响明显。 |
| 关键词: 结直肠肿瘤 肽库 药物疗法 自身抗体 |
| DOI：10.3724/SP.J.1008.2014.00085 |
| 投稿时间：2013-03-19修订日期：2013-12-05 |
| 基金项目:上海市登山计划重大课题（06DZ19503）。 |
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| Effect of chemotherapy on detection of colorectal cancer autoantibodies by phage display peptide method |
| LIU Yan, CHANG Wen-jun, CAO Guang-wen* |
(Department of Epidemiology, Faculty of Tropical Medicine and Public Health, Second Military Medical University, Shanghai Key Laboratory of Medical Biodefense, Shanghai 200433, China
*Corresponding author.) |
| Abstract: |
| Objective To evaluate the effect of chemotherapy on detection of colorectal cancer autoantibodies by phage display peptide method. Methods The five-phage peptide clones (No.95, No.149, No.174, No.396, and No.1009) with high discriminatory ability of colorectal cancer patients were selected as the study subjects. We compared the reactivity of autoantibodies against each of the five-phage peptide clones among 20 colorectal cancer patients receiving chemotherapy, 40 colorectal cancer patients receiving no chemotherapy, and 40 healthy controls by enzyme linked immunosorbent assay (ELISA). Results The seroreactivity of No.95 clone was significantly lower in the patients with chemotherapy than those without chemotherapy (P<0.05), and was similar between normal control group and patients with chemotherapy (P=0.074). For the seroreactivity of the other four clones (No.149, No.174, No.396, No.1009), there were significant differences between patients with chemotherapy and normal controls (all P<0.01). Conclusion The detection ability of No.95 clones for colorectal cancer autoantibodies is greatly influenced by chemotherapy, while chemotherapy shows no notable influence on the reactivity of other four-phage peptide clones. |
| Key words: colorectal neoplasms peptide library drug therapy autoantibody |
