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早年慢性应激对青春期病理性攻击大鼠空间学习记忆及海马脑源性神经营养因子、5-羟色胺的影响
张艳1,雷莉1,胡华1*,屈远1,吕小娟1,秦光成2,陈力学2
0
(1.重庆医科大学附属第一医院心理卫生中心,重庆 400016
2.重庆医科大学附属第一医院神经病学实验室,重庆 400016
*通信作者)
摘要:
目的 探索早年慢性应激对青春期病理性攻击大鼠空间学习记忆能力,及对海马脑源性神经营养因子(BDNF)、5-羟色胺(5-HT)水平的影响。方法 将20只雄性、出生后21 d 的Sprague-Dawley大鼠分为实验组、对照组,每组各10只。实验组大鼠采用孤养、昼夜颠倒、非奖赏性挫败、预激惹刺激、居住-入侵攻击等多种早年持续性应激方法持续到青春期。然后采用居住-入侵攻击实验检测两组青春期大鼠的攻击性,水迷宫实验观察其空间学习记忆能力,运用免疫组化方法检测大鼠海马内BDNF和5-HT水平。结果 (1)水迷宫实验:实验组大鼠在总路程、穿越目标区域次数、逃避潜伏期3项指标上与对照组差异有统计学意义(P<0.05),而中心区域路程/总路程比值与对照组比较差异无统计学意义(P>0.05)。(2)免疫组化结果显示:与对照组比较,实验组大鼠海马内BDNF和5-HT免疫阳性神经元数量均减少(P<0.05),BDNF和5-HT表达降低。结论 BDNF和5-HT可能参与了青春期病理性攻击大鼠海马空间学习记忆能力的调控,并在空间学习记忆方面起着重要调控作用。
关键词:  脑源性神经营养因子  血清素  攻击  空间学习记忆  早年慢性应激  青春期
DOI:
投稿时间:2013-03-26修订日期:2013-06-09
基金项目:重庆市自然科学基金(CSTC,2011jjA10104),重庆医科大学国家自然科学预研资助项目(NSFYY201111).
Effect of early life chronic stress on spatial learning and memory and hippocampus brain-derived neurotrophic factor and 5-HT in puberty pathological aggression rats
ZHANG Yan1,LEI Li1,HU Hua1*,QU Yuan1,L Xiao-juan1,QIN Guang-cheng2,CHEN Li-xue2
(1. Department of Psychiatry, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
2. Laboratory of Neurology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
*Corresponding author.)
Abstract:
Objective To explore the effect of early life chronic stress on spatial learning and memory and hippocampus brain-derived neurotrophic factor (BDNF) and 5-HT levels in puberty pathological aggression rats. Methods Twenty 21-day-old male rats were evenly randomized into 2 groups: experimental group and control group. Animals in the experimental group, from the early life till puberty, were given a series of stresses, including social isolation, reversed night and day, frustration test of non-reward, and resident intruder confrontations, etc. Resident intruder experiment was used to examine the aggressiveness of the animals; water maze experiments were performed to observe their spatial learning and memory. Immunohistochemistry method was used to test the expression of BDNF and 5-HT in the hippocampus. Results Morris water maze test showed that the total distance of the experimental group was significantly longer than that of the control group (P>0.05), and the experimental group had significantly less crossing times of hidden platform and escape latency compared with the control group (P<0.05); the ratios of central area distance/total distance were similar in the two groups. Immunohistochemistry findings showed that BDNF and 5-HT positive neurons were significantly less in the hippocampus of experimental group compared with the control group (P<0.05), and hippocampal BDNF and 5-HT expression in the experimental group was decreased. Conclusion BDNF and 5-HT may participate in the regulation of spatial learning and memory in the puberty pathological aggression rats, and they may play an important regulating role in spatial learning and memory.
Key words:  brain-derived neurotrophic factor  serotonin  aggression  spatial learning and memory  early life chronic stress  puberty