| 摘要: |
| 目的 研究中国南海小月柳珊瑚Menella kanisa中的化学成分。方法 应用正相硅胶、Sephadex LH-20凝胶、半制备反相高效液相色谱法(RP-HPLC)等多种分离手段对小月柳珊瑚Menella kanisa的乙醚提取物进行分离纯化,根据现代波谱技术结合文献报道进行结构鉴定。结果和结论 从南海小月柳珊瑚Menella kanisa中分离得到5个过氧化麦角甾, 分别鉴定为: 5α,8α-过氧化胆甾-6-烯-3β-醇(1)、(22E)-5α,8α-过氧化胆甾-6,22-二烯-3β-醇(2)、(22E,24R)-5α,8α-过氧化麦角甾-6,22-二烯-3β-醇(3)、5α,8α-过氧化麦角甾-6,24(28)-二烯-3β-醇(4)、24(E)-5α,8α-过氧化胆甾-24-乙基-6,24(28)-二烯-3β-醇(5)。这5个过氧化甾醇均为首次从该种珊瑚中分离得到。 |
| 关键词: 小月柳珊瑚 过氧化甾醇 天然产物 结构鉴定 |
| DOI： |
| 投稿时间：2013-04-10修订日期：2013-06-09 |
| 基金项目:国家自然科学基金面上项目(81172979), 国家高技术研究发展计划(“863”计划,2013AA092902). Suported by National Natural Science Foundation of China (81172979) and National High-Tech R&D Program (“863” Project, 2013AA092902). |
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| Research on 5α,8α-epidioxy sterols from the South China Sea gorgonian Menella kanisa |
| WANG Pan1,2,ZHOU Wei2,TANG Hua2,LIU Bao-shu2,SUN Peng2,ZHU Wen1,LUO Yan-ping1*,ZHANG Wen2* |
(1. The Environment and Plant Protection College, Hainan University, Haikou 570228, Hainan, China
2. Research Center for Marine Drugs, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
*Corresponding authors.) |
| Abstract: |
| Objective To investigate the chemical components of gorgonian Menella kanisa collected from the South China Sea. Methods The Et2O extract of gorgonian Menella kanisa was purified by repeated column chromatography on silica gel, Sephadex LH-20, and reversed-phase high-performance liquid chromatography (RP-HPLC). The structures of the obtained compounds were determined on the basis of spectroscopic analysis and compared with the reported data. Results and Conclusion Five 5α,8α-epidioxy sterols were isolated from gorgonian Menella kanisa collected from the South China Sea, and their structures were determined as: 5α,8α-epidioxy-cholest-6-en-3β-ol (1), (22E)-5α,8α-epidioxy-cholesta-6,22-dien-3β-ol (2), (22E,24R)-5α,8α-epidioxy-24-methyl-cholesta-6,22-dien-3β-ol (3), 5α,8α-epidioxy-24-methyl-cholesta-6,24(28)-dien-3β-ol (4), (24E)-5α,8α-epidioxy-24-ethyl-cholesta-6,24(28)-dien-3β-ol (5). All the five epidioxy sterols have been separated from the title Menella kanisa for the first time. |
| Key words: Menella kanisa epidioxy sterols natural product structural elucidation |
