| 摘要: |
| 目的 制备3种不同功能化的纳米金,并对其粒径、zeta电位、形态及稳定性进行评价。方法 采用化学还原法分别合成以聚乙烯吡咯烷酮(PVP)作为保护剂的纳米金(PVP-AuNPs)、脂质体修饰的纳米金(DODAB-AuNPs)、半胱胺修饰的纳米金(CA-AuNPs);采用动态光散射测定3种功能化纳米金的粒径及zeta电位;通过透射电镜观察纳米金的形态;采用紫外吸收法考察3种功能化纳米金在不同离子强度(加入同体积浓度分别为0.01、0.1、0.5、1 mol/L的NaCl, pH 7.2)和不同pH(1.0～14.0)下的稳定性。 结果 PVP-AuNPs、DODAB-AuNPs、CA-AuNPs的粒径分别为(15.0±3.1)、(22.7±6.1)、(18.0±4.6) nm,均为20 nm 左右;zeta电位分别为(-19.7±4.1)、(62.8±4.3)、(33.3±7.7) mV;紫外吸收法证明PVP-AuNPs及DODAB-AuNPs在高离子强度溶液及较广的pH环境下均有良好的稳定性,而CA-AuNPs对离子强度和pH环境的改变比较敏感,当加入的NaCl浓度为0.01 mol/L或pH值在4.5～6.5范围时能够维持理想的分散状态,反之,粒子间发生聚集。结论 3种功能化纳米金粒子具有纳米级球形结构和相对较高的稳定性,不同修饰后具有不同表面电位,可为药物传递载体的设计提供参考。 |
| 关键词: 金 纳米粒子 修饰 药物稳定性 药物载体 |
| DOI： |
| 投稿时间：2013-04-16修订日期：2013-07-12 |
| 基金项目: |
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| Synthesis of three kinds of surface-modified gold nanoparticles and comparison of their stability |
| YU Fei fei1,WANG Xin xia2,ZOU Hao1,ZHANG Guo qing2,ZHONG Yan qiang1* |
(1. Department of Pharmaceutics, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
2. Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China
*Corresponding author.) |
| Abstract: |
| Objective To synthesize three kinds of surface-modified gold nanoparticles and to compare their size distribution, zeta potential, surface morphology, and stability. Methods Poly (vinyl pyrrolidone) (PVP) stabilized gold nanoparticles (PVP-AuNPs), didodecyldimethylammonium bromide (DODAB, a cationic lipid) coated gold nanoparticles (DODAB-AuNPs), and cysteamine modified gold nanoparticles (CA-AuNPs) were successfully synthesized by chemical reduction method. The size distribution, zeta potential, and surface morphology of the three kinds of gold nanoparticles were characterized by dynamic light scattering (DLS) and transmission electron microscopy (TEM); and the stability of them was evaluated in various concentrations of sodium ions (0.01, 0.1, 0.5 and 1 mol/L NaCl; pH=7.2) and at different pH values (1.0-14.0) by UV-Vis absorption spectra. Results The mean diameters of PVP-AuNPs, DODAB-AuNPs, and CA-AuNPs were (15.0±3.1) nm, (22.7±6.1) nm, and (18.0±4.6) nm, respectively; and their zeta potentials were (-19.7±4.1), (62.8±4.3), and (33.3±7.7) mV, respectively. It was also found that PVP-AuNPs and DODAB-AuNPs were very stable in NaCl solution and different pH environments. However, CA-AuNPs solution was sensitive to concentration of sodium ions and pH value changes and it could maintain stable only when the concentration of NaCl was 0.01 mol/L or the pH value was within 4.5-6.5; otherwise there would be aggregation. Conclusion The three kinds of gold nanoparticles have a nano spherical shape and good stability, with different surface potentials when modified with different ligands; these findings provide reference for design of drug delivery carriers. |
| Key words: gold nanoparticles modification drug stability drug carriers |
