| 摘要: |
| 目的 运用快速液相-高分辨飞行时间质谱(RRLC-TOF/MS)技术鉴别淫羊藿大鼠给药后的入血成分及代谢产物,以探索淫羊藿可能的药效成分及体内代谢过程。 方法 色谱分离采用SHISEIDO MG C18 (3.0 mm×100 mm,3 μm) 色谱柱;流动相为乙腈(A相)和0.1％甲酸水溶液(B相),梯度洗脱:0～3 min(5%～19%A),3～13 min(19%～32%A),13～25 min(32%～52%A),25～30 min(52%～70%A);柱温25℃;流速0.6 mL/min,柱后分流比为31。质谱定性采用飞行时间质谱,电喷雾离子源(ESI),正离子模式,质量数扫描范围m/z 100～1 000。 结果 鉴别出淫羊藿中3种入血成分及4种代谢物。结论 所建方法快捷、有效,可用于淫羊藿体内成分鉴别,为进一步的药效试验提供了参考和依据。 |
| 关键词: 淫羊藿 入血成分 代谢产物 飞行时间质谱 |
| DOI： |
| 投稿时间：2013-06-05修订日期：2013-07-01 |
| 基金项目:无 |
|
| RRLC-TOF/MS in identification of plasma constituents and metabolites of epimedium in rats after intragastric administration |
| L Lei,ZHAO Liang,ZHANG Hai,LI Yue-yue,ZHANG Guo-qing* |
(Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China
*Corresponding author.) |
| Abstract: |
| Objective To identify the constituents and metabolites of epimedium in rat plasma by rapid-resolution liquid chromatography-time of flight mass spectrometry (RRLC-TOF/MS) after intragastric administration, so as to explore the active ingredients and metabolites of epimedium in vivo. Methods The separation was performed on a SHISEIDO MG C18 reverse phase column (3.0 mm×100 mm,3 μm)．The mobile phase consisted of water containing 0.1% formic acid and acetonitrile was used as gradient elute．The flow rate was 0.6 mL/min, post-column split ratio was 31, and the temperature of column was 25℃. Time-of-flight mass spectrometer(TOF/MS) and electrospray ion source (ESI) were applied for qualitative analysis under positive ion mode, with the mass scan range being m/z 100-1 000. Results Three prototype constituents and 4 metabolites of epimedium were identified in rat plasma. Conclusion A rapid and efficient RRLC-TOF/MS method has been established for studying the constituents and metabolites of epimedium in vivo, which provides a reference for further pharmacodynamics experiments. |
| Key words: Epimedium brevicornum prototype constituents metabolites time of flight/mass spectrometry |
