| 摘要: |
| 目的 研究牛樟芝能否延长易卒中的自发性高血压大鼠的生存时间，并运用蛋白质组学方法对其作用机制进行探讨。 方法 先选取80只雄性易卒中的自发性高血压大鼠，随机分为给药组和对照组（每组40只），给药组每天用牛樟芝（150 mg/kg）灌胃治疗，记录卒中大鼠的自然死亡时间。另外再选取6只易卒中的自发性高血压大鼠，随机分为给药组和对照组（每组3只），给药组给予牛樟芝灌胃治疗（150 mg/kg），连续90 d后取大鼠脑组织进行蛋白质组学研究，用WKY大鼠作为正常对照，对用药前后产生的差异蛋白点进行质谱鉴定，并用蛋白质印迹法进一步验证组学的鉴定结果。 结果 牛樟芝延长了易卒中的自发性高血压大鼠的生存时间（P<0.05），蛋白质组学鉴定结果显示牛樟芝上调了脑组织抗氧化酶谷胱甘肽巯基转移酶（GST）和超氧化物歧化酶（SOD）的表达（P<0.05），这一结果得到了蛋白质印迹法的验证。对脑组织氧化应激水平的研究发现机体的总抗氧化能力增强[T-AOC：（66.48±16.17） U/g vs （124.75±28.43） U/g， P<0.05），表现在GST和SOD的酶活性增加[GST：（40.33±5.24） U/mg vs （70.50±6.24） U/mg， P<0.05； SOD：（109.25±23.61） U/mg vs （192.60±23.95） U/mg，P<0.05]，而氧化应激产物丙二醛含量减少[（3.96±0.45） nmol/mg vs （2.04±0.31） nmol/mg，P<0.05]。 结论 长期服用牛樟芝能延长易卒中的自发性高血压大鼠的生存时间，这可能与其增加脑组织抗氧化酶的表达、减轻氧化应激的损伤有关。 |
| 关键词: 牛樟芝 易卒中的自发性高血压大鼠 卒中 氧化性应激 脑 蛋白质组学 |
| DOI：10.3724/SP.J.1008.2014.00043 |
| 投稿时间：2013-06-27修订日期：2013-09-04 |
| 基金项目:军队中医药科研专项课题（10ZYZ204）。 |
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| Effect of Antrodia cinnamomea on brain proteomics in stroke-prone spontaneously hypertensive rats |
| CHENG Li-juan1,2, LEI Hong2, GUO Qi2, WANG Yan-zong2* |
(1. Department of Pharmacology, Hebei North University, Zhangjiakou 075000, Hebei, China;
2. Division of Traditional Chinese Medicine Testing, Institute for Drug and Instrument Control, The Joint Logistics Department, PLA Beijing Military Area Command, Beijing 100071, China
*Corresponding author.) |
| Abstract: |
| Objective To explore whether Antrodia cinnamomea can prolong the survival time of stroke-prone spontaneously hypertensive rats, and to investigate the underlying mechanisms from the proteomics perspective. Methods A total of 80 male stroke-prone spontaneously hypertensive rats were randomly divided into two groups (control group and Antrodia cinnamomea treated group, n=40). The animals were intragastrically given Antrodia cinnamomea (150 mg/kg). Then the death of the animals in the two groups was observed. Another 6 stroke-prone spontaneously hypertensive rats were randomly divided into drug treatment group and control group, with the drug treatment group intragastrically given Antrodia cinnamomea (150 mg/kg), and the brain proteomics were compared between the two groups 90 days later, with WKY rats used as normal controls. The differentially expressed proteins before and after drug treatment were subjected to mass spectrometry analysis, and the results of mass spectrometry analysis were further confirmed by Western blotting analysis. Results Antrodia cinnamomea significantly prolonged the survival time of stroke-prone spontaneously hypertensive rats (P<0.05), and proteomics results showed that Antrodia cinnamomea increased the expression of glutathione S-transferase (GST) and superoxide dismutase (SOD), which were also confirmed by Western blotting analysis. Further study revealed that the total anti-oxidative capability (T-AOC) of the animals was increased (T-AOC: [66.48±16.17] U/g vs [124.75±28.43] U/g, P<0.05), which was manifested by the increased activity of GST and SOD (GST: [40.33±5.24] U/mg vs [70.50±6.24] U/mg, P<0.05; SOD: [109.25±23.61] U/mg vs [192.60±23.95] U/mg, P<0.05), and decreased level of malondialdehyde ([3.96±0.45] nmol/mg vs [2.04±0.31] nmol/mg, P<0.05). Conclusion Long-term treatment with Antrodia cinnamomea can prolong the lifespan of stroke-prone spontaneously hypertensive rats, which might be related to the increased activity of anti-oxidative enzymes and the decreased oxidative damage. |
| Key words: Antrodia cinnamomea stroke-prone spontaneously hypertensive rats stroke oxidative stress brain proteomics |
