【打印本页】 【下载PDF全文】 【HTML】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览 2194次   下载 2467 本文二维码信息
码上扫一扫!
骨髓间充质干细胞抑制肾癌A498细胞增殖及侵袭能力
邓震,刘军,朱凌峰,陈书尚,吴卫真,谭建明*
0
(南京军区福州总医院泌尿外科, 福州 350001
*通信作者)
摘要:
目的 探讨骨髓间充质干细胞对肾癌细胞系A498增殖和侵袭能力的影响。方法 将骨髓间充质干细胞及人成纤维细胞分别与肾癌A498细胞共培养, 利用流式细胞仪测定细胞周期, 检测骨髓间充质干细胞及人成纤维细胞对A498细胞增殖能力的影响;Transwell侵袭实验分析肾癌A498细胞体外侵袭能力;qRT-PCR检测共培养后肾癌A498细胞Snail和E-cadherin基因的表达。结果 骨髓间充质干细胞可明显抑制A498细胞的增殖及侵袭能力(P<0.05);qRT-PCR结果表明, 与骨髓间充质干细胞共培养后, A498细胞中Snail基因表达下调(P<0.05), 而E-cadherin基因表达上调(P<0.05)。成纤维细胞对A498细胞所起的作用与骨髓间充质干细胞相反。结论 骨髓间充质干细胞体外可抑制A498细胞的增殖和侵袭。
关键词:  肾肿瘤  骨髓  间质干细胞  细胞增殖  肿瘤侵袭
DOI:10.3724/SP.J.1008.2015.00488
投稿时间:2014-09-11修订日期:2015-01-21
基金项目:福建省科技厅基金(2012D022).
Inhibitory effect of bone marrow mesenchymal stem cells against proliferation and invasion of human renal cell carcinoma A498 cells
DENG Zhen,LIU Jun,ZHU Ling-feng,CHEN Shu-shang,WU Wei-zhen,TAN Jian-ming*
(Department of Urology, Fuzhou General Hospital, PLA Nanjing Military Area Command, Fuzhou 350001, Fujian, China
*Corresponding author)
Abstract:
Objective To study the inhibitory effect of bone marrow mesenchymal stem cells (BM-MSCs) on the proliferation and invasion of human renal cell carcinoma cell line A498. Methods Human renal cell carcinoma cell line A498 cells were co-cultured with BM-MSCs or human dermal fibroblasts-adult (HDF-a) cells separately, and then cell proliferation was detected by flow cytometry and cell invasion ability was detected by transwell assay in each group. qRT-PCR was used to exam Snail and E-cadherin mRNA expression in A498 cells, HDF-a-treated A498 cells and BM-MSCs-treated A498 cells. Results BM-MSCs greatly inhibited the proliferation and invasion of A498 cells (P<0.05). qRT-PCR showed that co-culture with BM-MSCs significantly down-regulated Snail mRNA expression (P<0.05) and up-regulated E-cadherin mRNA expression (P<0.05) in A498 cells. Compared with BM-MSCs, HDF-a played an opposite role as for the above items. Conclusion BM-MSCs play an important role in the proliferation and invasion of LNCaP cells, which may cast new light for the biotherapy of human renal cell carcinoma cancer.
Key words:  kidney neoplasms  bone marrow  mesenchymal stem cells  cell proliferation  neoplasm invasiveness