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| 聚乙二醇修饰的聚精氨酸基因载体的构建及其体外评价 |
| 台宗光1,2,孙霖2,朱全刚1,张玮1,3,王晓宇1,戴子渊1,张丽娟1,高申1* |
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(1. 第二军医大学长海医院药学部, 上海 200433;
2. 解放军92330部队医院, 青岛 266102;
3. 解放军522医院药材科, 洛阳 471003
*通信作者) |
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| 摘要: |
| 目的 利用聚乙二醇(PEG)修饰非病毒基因载体聚精氨酸(PLR),考察PEG修饰对PLR的细胞毒性和PLR介导的RNA干扰效率的影响。方法 利用1HNMR鉴定合成的PLR-PEG的结构,确定PEG的修饰度,利用凝胶电泳表征载体对siRNA的包裹能力,在前列腺癌干细胞模型细胞(RC-92a/hTERT)上考察PLR-PEG的细胞毒性,考察PLR-PEG/siRNA复合物的细胞摄取及相关基因的干扰效率。结果 通过结构鉴定确定PLR-PEG合成成功；细胞毒性实验表明PEG修饰可以降低PLR的毒性；PEG修饰会降低PLR/siRNA复合物的细胞摄取,高N/P时对细胞摄取的影响不大；PEG修饰也会降低PLR介导的RNA干扰效率,但PEG修饰度在一定范围内对干扰效率的影响比较小。结论 PEG修饰的PLR作为基因载体在前列腺癌干细胞的基因治疗中有一定的应用前景。 |
| 关键词: 聚精氨酸 聚乙二醇 基因载体 细胞毒性 基因干扰效率 前列腺癌干细胞 |
| DOI：10.3724/SP.J.1008.2014.01088 |
| 投稿时间：2014-02-20修订日期：2014-08-30 |
| 基金项目:国家自然科学基金(81272819，81172514，81101658). |
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| Preparation of polyethylene glycol-modified polyarginine for gene delivery and in vitro evaluation |
| TAI Zong-guang1,2,SUN Lin2,ZHU Quan-gang1,ZHANG Wei1,3,WANG Xiao-yu1,DAI Zi-yuan1,ZHANG Li-juan1,GAO Shen1* |
(1. Department of Pharmacy, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;
2. Hospital of No. 92330 Troop of PLA , Qingdao 266102, Shandong, China;
3. Department of Pharmacy, No. 522 Hospital of PLA, Luoyang 471003, Henan, China
*Corresponding authors) |
| Abstract: |
| Objective To modify polyargine (PLR) with polyethylene glycol (PEG) and to observe the effect of PEG modification on PLR cytotoxicity and efficiency of PLR-mediated RNA interference. Methods 1HNMR was used to characterize PLR-PEG and gel electrophoresis was adopted to determine the siRNA-packing capacity of PLR-PEG. The cytotoxicity of PLR-PEG, cellular uptake and RNA interference efficiency of PLR-PEG/siRNA complexes were investigated using prostate cancer stem-like cells(RC-92a/hTERT). Results 1HNMR results showed the successful synthesis of PLR-PEG.It was found that PEG modification decreased cytotoxicity of PLR and reduced cellular uptake of PLR/siRNA complexes, but the reduction of cellular uptake was limited when N/P was high. The modification also inhibited the efficiency of PLR-mediated RNA interference, but the influence of PEG modification was not notable within a certain range. Conclusion PEG -modified PLR may be a promising vector for gene therapy targeting prostate cancer stem cells. |
| Key words: polyarginine polyethylene glycol gene delivery vector cytotoxicity gene interference efficiency prostate cancer stem cells |
