| 摘要: |
| [摘要] 目的 分析乳腺纤维腺瘤病人与乳腺浸润性导管癌病人在血清代谢组学上的差异，探讨术前通过代谢特征来辨别良恶性肿瘤的可能性，从而为临床诊断提供代谢依据。方法 通过核磁共振1H NMR技术检测乳腺纤维腺瘤病人与乳腺浸润性导管癌病人血清代谢组特征，分别采用主成分分析(principle component analysis，PCA)和正交最小偏二乘—判别法 (orthogonal signal correction partial least squares discrimination analysis ，OPLS-DA)分析纤维腺瘤与浸润性导管癌两者之间的代谢差异。结果 PCA和OPLS-DA分析显示两者有明显的代谢差异。对比乳腺纤维腺瘤患者血清代谢特征，发现在恶性肿瘤浸润性导管癌患者血清中脂类物质、乳酸、磷脂酰胆碱/甘油磷脂酰胆碱的含量是升高的，而谷氨酰胺、牛磺酸、肌酸和葡萄糖是降低的。这些代谢物的改变涉及糖代谢，脂质代谢和氨基酸代谢等多个代谢通路的改变。结论 乳腺纤维腺瘤病人与乳腺浸润性导管癌病人在血清代谢水平上有明显差异，这些具有显著性变化的代谢物可作为潜在血清代谢标志物，为临床区分良恶性肿瘤并确定治疗方案提供参考。 |
| 关键词: 核磁共振 乳腺纤维腺瘤 浸润性导管癌 代谢组学 |
| DOI：10.3724/SP.J.1008.2014.00879 |
| 投稿时间：2013-12-05修订日期：2014-01-06 |
| 基金项目:国家自然科学基金 |
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| The 1H NMR-based serum metabonomics study of breast fibroadenoma and breast invasive ductal carcinoma patients |
| Liuying,Jitianxing,Lijiangchao,Wanglijing,Yangyongxia |
| (Guangdong Pharmaceutical University;Department of clinical laboratory, the second affiliated hospital of Guangzhou Medical University) |
| Abstract: |
| [Abstract] Objective To research the serum metabonomic difference between breast fibroadenoma patients and breast invasive ductal carcinoma patients based on 1H NMR spectroscopy and to offer reliable biochemical information for clinical diagnosis. Methods The serum metabolic spectra of breast fibroadenoma patients and breast invasive ductal carcinoma patients were collected. The data were processed using Simca-p software and analyzed with principle component analysis(PCA) and orthogonal signal correction partial least squares discrimination analysis(OPLS－DA). Results There were clear separation between breast fibroadenoma patients and breast invasive ductal carcinoma patients through analysis of PCA and OPLS-DA .And the level of lipid, lactate and PC/GPC was higher in breast invasive ductal carcinoma patients than in breast fibroadenoma patients, but glutamine, taurine, creatine, glucose were comparatively reduced in various degree. The potential biomarkers were involved in many metabolic pathways including glucose metabolism, lipid metabolism and amino acid metabolism. Conclusion Distinct difference existed in serum metabolomic profiles between breast adenoma patients and breast invasive ductal carcinoma patients. These changed metabolites may serve as the potential biomarkers to help the differentiation malignant tumor from benign and to provide reference to make therapeutic schedule. |
| Key words: 1H NMR breast fibroadenoma breast invasive ductal carcinoma matabonomics |
