| 摘要: |
| 目的 研究不同病理级别肾细胞癌中差异表达的长链非编码RNAs（lncRNAs），找到其中一种lncRNA——RCRF与肾癌患者临床病理资料的关系，从而判断肾癌预后。方法 取6例肾细胞癌原发灶肿瘤组织，3例病理分级为FuhrmanⅠ级，另3例为Fuhrman Ⅲ~Ⅳ级，采用lncRNA芯片技术，筛选差异表达的lncRNAs。另取40例肾细胞癌患者的癌组织及癌旁正常组织，22例病理分级为FuhrmanⅠ~Ⅱ （低级别），18例为Fuhrman Ⅲ~Ⅳ （高级别），利用实时定量PCR技术检测在癌及癌旁非肿瘤组织中RCRF的表达，并研究RCRF与临床分期、病理分级、淋巴结转移、远处转移之间的关系。结果 筛选出320个与肾细胞癌病理级别相关的差异表达lncRNAs。肾癌组织尤其是高级别肾癌组织中RCRF的相对表达上调（P<0.01）。RCRF表达与肾癌病理分级、淋巴转移及远处转移有关（P<0.05）。结论 RCRF在高级别肾癌组织中表达上调，并且影响肾癌患者的预后。 |
| 关键词: 肾肿瘤 肾细胞癌 长链非编码RNA RCRF 预后 |
| DOI：10.3724/SP.J.1008.2014.00566 |
| 投稿时间：2014-01-02修订日期：2014-03-12 |
| 基金项目: |
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| Renal carcinoma related factor is up-regulated in high Fuhrman grade renal cell carcinoma tissues and affects prognosis of patients |
| SONG Shang-qing,WU Zhen-jie,WANG Cheng,LIU Bing,WANG Lin-hui* |
(Department of Urology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
*Corresponding authors.) |
| Abstract: |
| Objective To identify differentially expressed long non-coding RNAs (lncRNAs) in different Fuhrman grade renal cell carcinoma (RCC) tissues, and to investigate the association of one of these lncRNAs-renal carcinoma related factor (RCRF) with the clinicopathologic features of RCC patients, helping the prognosis of RCC patients. Methods A total of 6 RCC tumor tissues were used for lncRNA chip screening, with 3 being FuhrmanⅠgrade and the rest being Fuhrman Ⅲ-Ⅳ grade. Then another 40 RCC tumor tissues (22 FuhrmanⅠ-Ⅱgrade and 18 Fuhrman Ⅲ-Ⅳ grade) and the matched adjacent noncancerous tissues were subjected to qPCR examination for RCRF expression, and the relationship of RCRF expression with the clinical stage, tumor grade, lymph node metastasis and distant metastasis was analyzed. Results A total of 320 differentially expressed lncRNAs were screened out from RCC tumor tissues. Compared with the adjacent normal tissues, RCC tissues, especially the high grade ones, had significantly up-regulated RCRF expression(P<0.01). It was also found that RCRF expression was associated with the pathological grade, lymph node metastasis and distant metastasis of RCC (P<0.05). Conclusion RCRF is up-regulated in high Fuhrman grade tissues and may affect the prognosis of RCC patients. |
| Key words: kidney neoplasms renal cell carcinoma long non-coding RNAs RCRF prognosis |
