| 摘要: |
| 细胞培养稳定核素标记（stable isotope labeling by amino acids in cell culture，SILAC）技术是一种利用稳定核素标记细胞内蛋白并根据质谱丰度比率进行定量的体内代谢标记技术。结合不同的蛋白亲和富集方式，可以获得较高灵敏度和准确性的蛋白定量信息。本文综述了SILAC技术的发展历史、原理、特点以及应用，着重介绍了其在研究蛋白质与小分子间的相互作用、定量蛋白组学以及蛋白翻译后修饰等方面的应用进展。 |
| 关键词: 细胞培养稳定核素标记 亲和富集 质谱 定量蛋白组学 |
| DOI：10.3724/SP.J.1008.2014.00892 |
| 投稿时间：2014-02-25修订日期：2014-03-20 |
| 基金项目:国家自然科学基金青年科学基金（31000079），国家重点基础研究发展计划（“973”计划，2013CB531602），国家自然科学基金重点项目（81330083）. |
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| Stable isotope labeling by amino acids in cell culture：development and application |
| LÜ Quan-zhen,YAN Lan*,JIANG Yuan-ying* |
| (New Drug Research Center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| Stable isotope labeling by amino acids in cell culture (SILAC) is an in vivo metabolic labeling technique based on the abundance ratio of mass spectrometry. Combined with different methods of proteins affinity enrichment, SILAC can obtain sensitive, accurate and quantitative information of proteins. This article reviewed the history, rationale, characteristics and application of SILAC, with special focus on its role in studying protein interactions with small molecules, quantitative proteomics and post-translational modification of proteins. |
| Key words: stable isotope labeling by amino acids in cell culture affinity enrichment mass spectrometry quantative proteomics |
