【打印本页】 【下载PDF全文】 【HTML】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览 1756次   下载 2062 本文二维码信息
码上扫一扫!
白介素13在胆管闭锁和先天性胆管扩张患儿外周血及肝组织中的表达
刘磊,田洪孝*
0
(泰安市妇幼保健院外一科, 泰安 271000
*通信作者)
摘要:
【】 目 的 研究胆道闭锁(BA)患儿血清及肝脏中白介素-13(IL-13)的表达及意义。方 法 选取13例BA与10例胆管扩张症(CBD)患儿血清和肝脏标本进行对比研究。应用酶联免疫吸附试验检测血清中IL-13的表达水平。荧光定量RT-PCR技术和免疫印迹技术分别检测肝脏组织中IL-13 mRNA及蛋白表达水平。结 果 BA组血清中IL-13表达明显低于CBD组(154.5±18.23 pg/ml vs. 268.2±19.30 pg/ml,p<0.01)。BA组肝组织中IL-13 mRNA表达明显低于CBD组(0.0150±0.0021 vs. 0.0320±0.0050,p=0.0220)。BA组肝组织中IL-13 蛋白表达亦明显低于CBD组(0.3446±0.0150 vs. 0.5765±0.0215,p=0.0122)。
关键词:  胆道闭锁  白介素13  先天性胆管扩张
DOI:10.3724/SP.J.1008.2015.00808
投稿时间:2014-12-15修订日期:2015-06-15
基金项目:
Expression of interleukin-13 in peripheral blood and liver of infants with biliatry atresia or congenital biliary dilatation
LIU Lei,TIAN Hong-xiao*
(Department of Surgery, Maternity and Child Care Hospital of Taian, Taian 271000, Shangdong, China
*Corresponding author)
Abstract:
Objective To study the expression pattern of interleukin-13 (IL-13) in the sera and liver tissue of biliary atresia. Methods 13 infants with biliatry atresia (BA) were studied. 10 children with congenital biliary dilatation (CBD) were studied as a control. Both the BA and CBD were confirmed at operation and histological examination on the liver tissue of biopsy. The sera level of IL-13 was measured by enzyme-linked immunosorbent assay. The expression of IL-13 mRNA in liver tissue was detected with Real-time quantitative reverse transcriptase-polymerase chain reaction. The expression of IL-13 protein in liver tissue was examined by western blotting. Results The IL-13 level in sera of BA was significantly lower than that of CBD group (154.5±18.23 pg/ml vs. 268.2±19.30 pg/ml,p<0.01). Compared with CBD group, the expression of IL-13 mRNA in liver tissue was significantly lower in BA (0.0150±0.0021 vs. 0.0320±0.0050, p=0.0220), and the expression of IL-13 protein was decreased (0.3446±0.0150 vs. 0.5765±0.0215, p=0.0122). Conclusions The expression of IL-13 in the sera and liver tissue of BA is significantly elevated. It may play a key role in the pathogenesis of BA and is worthy of further investigation.
Key words:  biliary atresia  interleukin-13  congenital biliary dilatation