| 摘要: |
| 目的 研究RIZ1基因在不同级别脑胶质瘤组织中的蛋白表达水平以及对患者预后的影响。方法 应用免疫组织化学染色检测RIZ1和Ki-67在51例不同级别星型胶质细胞瘤中的蛋白表达水平。利用统计学方法分析RIZ1表达水平与患者临床病理学特征和预后的相关性。结果 免疫组化染色显示RIZ1蛋白表达主要分布在细胞浆,而Ki-67蛋白主要位于细胞核；低级别胶质瘤中RIZ1的染色程度显著高于高级别胶质瘤,分别为48.83±4.34% vs. 7.55±4.39%,差异具有显著性意义(P<0.0001)；RIZ1与Ki-67的表达水平成明显负相关,相关系数r=－0.8130,P<0.0001。临床病理资料统计分析显示RIZ1的表达与肿瘤级别和患者年龄负相关(P<0.05),Kaplan–Meier生存分析显示RIZ1的表达水平与患者无进展生存期正相关(P < 0.0001)；Kaplan-Meier单因素分析提示RIZ1的高表达和Ki-67的低表达与患者预后正相关,而将单因素分析的结果中P <0.2的因素纳入多因素COX回归分析发现,RIZ1的高表达与胶质瘤患者预后良好有显著性关系。结论 RIZ1的表达水平随着胶质瘤病理级别的增高而减少,并与患者的预后明显相关,RIZ1是胶质瘤发病过程中的潜在抑癌癌基因。 |
| 关键词: RIZ1 胶质瘤 预后 |
| DOI：10.3724/SP.J.1008.2014.00847 |
| 投稿时间：2014-03-14修订日期：2014-05-15 |
| 基金项目:] 国家自然科学基金(30930094),国家自然科学青年基金(81001118)上海长征医院青年启动基金(2012CZQN05)。Supported by National Natural Science Foundation of China (30930094), National Natural Science Foundation of China (81001118)Foundation for Youths of Changzheng Hospital(2012CZQN05) |
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| A study of RIZ1 expression in gliomas and its association with patient prognosis1．Department of Neurosurgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China. |
| 张晨冉,wu xiaojun,hu liuhua,he hua,jiang lei,hu guohan,ding xuehua,lu yicheng |
| (Changzheng Hospital, Second Military Medical University) |
| Abstract: |
| Objective To investigate the expression level of RIZ1 and Ki-67 in astrocytomas of different grades and its association with patient prognosis. Methods The expression of RIZ1 and Ki-67 in glioma tissues were evaluated immunohistochemically in 51 patients with gliomas. The correlation of RIZ1 immunoreactivity with clinicopathological featurees and the prognostic value of RIZ1 in patients were also analyzed. Results Immunohistochemistry showed a decrease in RIZ1 labeling index in high grade gliomas versus low grade gliomas (7.55±4.39% vs 48.83±4.34%,P<0.0001). There was a negative correlation between RIZ1 and Ki-67 immunoreactivity (Spearman r =－0.8130, P<0.0001). Clinicopathological evaluation suggested that RIZ1 expression was negatively associated with tumor grade and patient age (P<0.05). Kaplan–Meier survival analysis revealed a significantly positive correlation between levels of RIZ1 immunoreactivity and PFS (progress free survival, PFS). Univariate survival analysis revealed significant relationships between expression of RIZ1, expression of Ki-67, and prognosis. Multivariate analysis using the Cox proportional hazards model for all variables included in univariate analysis revealed that high RIZ1 expression was independent prognostic factors for patients with gliomas. These results suggest that RIZ1 expression may be a key prognostic index for glioma patient survival. Conclusion: We conclude that high RIZ1 labeling index indicates lower grade of gliomas, and associated with increased patients’ PFS and overall survival (OS). RIZ1 may be a potential tumor suppressor in the development of gliomas. |
| Key words: RIZ1 glioma prognosis |
