| 摘要: |
| 目的 探讨原发性肝癌(HCC)患者外周血CD133+/CD105+细胞表达水平及其与临床预后的关系。方法 用流式细胞仪检测80例原发性肝癌患者和20例健康者CD45-/CD133+/CD105+细胞比例, 分析其与年龄、性别、临床分期、组织学分化程度、淋巴结转移及手术预后的关系, 采用Kaplan-Meier方法进行生存率分析,应用Cox比例风险模型进行多因素分析。结果 原发性肝癌患者外周血CD45-/CD133+/CD105+细胞水平较术后及健康对照组升高, 差异有统计学意义(P=0.003、P=0.000)。术后复发组术前外周血CD45-/CD133+/CD105+细胞水平高于术后未复发组,差异有统计学意义(P=0.015)。患者外周血CD45-/CD133+/CD105+细胞比例与临床分期、分化程度、有无淋巴结转移密切相关(均P<0.05),与年龄、性别无关。CD133与CD105的表达呈正相关(r=0.846, P<0.05)。患者外周血CD45-/CD133+/CD105+细胞比例> 0.5%者术后生存率较细胞比例≤0.5%者下降(P<0.05), CD45-CD133+细胞比例> 1.1%者术后生存率较细胞比例≤1.1%者降低(P<0.05), CD45-CD105+细胞比例> 36%者术后生存率较细胞比例≤36%者降低(P<0.05), 外周血CD45-/CD133+/CD105+细胞表达水平、分化程度、有无淋巴结转移是影响肝癌预后的独立因素。结论 肝癌患者外周血CD45-/CD133+/CD105+细胞表达水平明显升高并且与肝癌的恶性程度密切相关。外周血CD45-/CD133+/CD105+细胞表达水平降低是肝癌患者预后良好的独立因素, 是潜在的抗癌靶标。 |
| 关键词: 肝肿瘤 CD105 CD133 流式细胞术 预后 |
| DOI：10.3724/SP.J.1008.2014.01382 |
| 投稿时间：2014-05-15修订日期：2014-10-28 |
| 基金项目:安徽省科技攻关项目(1301zc04065). |
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| Expression of CD133 and CD105 on cells in peripheral blood of patients with primary hepatocellular carcinoma |
| XU Yong,WU Li-sheng,FANG Sheng,WANG Hong* |
(Department of Minimally Invasive Surgery, The Third Affiliated Hospital of Anhui Medical University, Hefei 230001, Anhui, China
*Corresponding author) |
| Abstract: |
| Objective To investigate the relationship of CD133+ and CD105+ cells in the peripheral blood with the clinical prognosis of patients with primary hepatocellular carcinoma (HCC). Methods The proportions of CD45-CD133+CD105+ cells were determined in 80 primary HCC patients and 20 healthy subjects by flow cytometry, and the correlation of the proportion with age, gender, clinical stage, histological differentiation, lymph node metastasis, and surgical outcomes were analyzed. The survival rate was analyzed by Kaplan-Meier curves, and multivariate analysis was performed using Cox proportional hazards model. Results The proportion of CD45-CD133+CD105+ cells in the peripheral blood of primary HCC patients was significantly higher compared with those in the postoperative patients and healthy controls (P=0.003, P=0.000). The proportion of preoperative CD45-CD133+CD105+ cells in the postoperative recurrent group was significantly higher than those in the non-recurrent group (P=0.015). The proportion of CD45-CD133+CD105+ cells in the peripheral blood of primary HCC patients was significantly associated with the clinical stage, degrees of differentiation, and presence/absence of lymph node metastasis (P<0.05), and not associated with age or gender. CD133 expression was positively correlated with CD105 expression (r=0.846, P<0.05). Postoperative survival rate was significantly lower in patients with CD45-CD133+CD105+ proportion > 0.5% compared with ≤ 0.5% (P<0.05). The postoperative survival rate of patients with CD45-CD133+ cell proportion > 1.1% was significantly lower than that with CD45-CD133+ cell proportion ≤1.1% (P<0.05). The postoperative survival rate of patients with CD45-CD105+ proportion > 36% was significantly lower than those with CD45-CD105+ proportion ≤ 36% (P<0.05). Peripheral blood CD45-CD133+CD105+ cell proportion, degree of differentiation, and presence/absence of lymph node metastasis were the independent factors influencing HCC prognosis. Conclusion Proportion of CD45-CD133+CD105+ cells in the peripheral blood is greatly increased in HCC patients, which is closely associated with the degree of malignancy of HCC. Decreased CD45-CD133+CD105+ cell proportion in the peripheral blood is an independent favorable prognostic factor for HCC patients, and targeted lowering of CD45-CD133+CD105+ cell proportion in the peripheral blood of primary HCC patients may represent a new treatment. |
| Key words: primary hepatic carcinoma CD105 CD133 flow cytometry prognosis |
