| 摘要: |
| 目的 探讨重庆汉族男性载脂蛋白B基因(apolipoprotein B, ApoB)MspⅠ、XbaⅠ、EcoRⅠ位点的多态性与血脂水平的关系。方法 采用基因芯片技术,检测血脂异常组157人和健康对照组180人载脂蛋白B基因MspⅠ、XbaⅠ、EcoRⅠ位点的多态性,并分析其与血脂水平的关系。结果 血脂异常组和健康对照组组内比较,MspⅠ 位点M+M-基因型的总胆固醇(TC)水平高于M+M+基因型[异常组:(6.54±0.58) vs (5.58±0.83) mmol/L, P<0.01; 对照组:(5.43±0.17) vs (4.39±0.62) mmol/L, P<0.01)];XbaⅠ位点X+X-基因型的高密度脂蛋白胆固醇(HDL-C)水平和低密度脂蛋白胆固醇(LDL-C)水平分别低于和高于X-X-基因型[异常组HDL-C:(1.08±0.27) vs (1.22±0.44) mmol/L, P=0.03; LDL-C:(3.88±0.63) vs (3.46±0.83) mmol/L, P=0.01; 对照组HDL-C:(1.31±0.43) vs (1.48±0.37) mmol/L, P=0.04; LDL-C:(3.19±0.54) vs (2.94±0.59) mmol/L, P=0.02];EcoRⅠ位点E+E-基因型的HDL-C水平低于E+E+基因型[异常组:(1.01±0.18) vs (1.21±0.43) mmol/L, P=0.01; 对照组:(1.27±0.20) vs (1.47±0.40) mmol/L, P=0.03]。血脂异常组和对照组组间比较,异常组X+X-基因型HDL-C 水平和LDL-C水平分别低于和高于对照组X+X-基因型[HDL-C:(1.08±0.27) vs (1.31±0.43) mmol/L, P=0.01; LDL-C:(3.88±0.63) vs (3.19±0.54) mmol/L, P<0.01];异常组E+E-基因型HDL-C水平低于对照组E+E-基因型[(1.01±0.18) vs (1.27±0.20) mmol/L, P<0.01]。多元线性回归分析结果显示,MspⅠ多态性与TC呈正相关,XbaⅠ多态性与HDL-C呈负相关、与LDL-C呈正相关。结论 ApoB基因MspⅠ和XbaⅠ多态性对血脂水平有一定的影响,M+M-基因型有使TC水平升高的趋势,X+X-基因型有使HDL-C水平降低、LDL-C水平升高的趋势。 |
| 关键词: 载脂蛋白B类 基因多态性 脂类 男人 |
| DOI：10.3724/SP.J.1008.2015.00966 |
| 投稿时间：2014-12-12修订日期：2015-02-28 |
| 基金项目:国家自然科学基金(81202274). |
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| Relationship between ApoB gene MspⅠ/XbaⅠ/EcoRⅠ polymorphisms and serum lipid level in male Han population in Chongqing, China |
| JIN Ya-na1,ZHOU Li1,TANG Min2,ZHANG Ming-jun3,TANG Xiao-jun1* |
(1. Department of Epidemiology, School of Public Health and Management, Chongqing Medical University, Chongqing 400016, China;
2. Department of Laboratory Diagnosis, College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China;
3. Department of Clinical Laboratory, First People's Hospital of Jiulongpo District, Chongqing 400016, China
*Corresponding author) |
| Abstract: |
| Objective To explore the relationship between apolipoprotein B (ApoB) gene MspⅠ/XbaⅠ/EcoRⅠ polymorphisms and the levels of serum lipid in male Han population in Chongqing. Methods The ApoB gene MspⅠ/XbaⅠ/EcoRⅠpolymorphisms were detected by gene chip technology in 157 dyslipidemia cases and 180 healthy controls; and their relationship with serum lipids was analyzed in the dyslipidemia group and the controls. Results In both dyslipidemia group and control group, the total cholesterol (TC) levels with M+M- genotype at MspⅠ locus were significantly higher than those with M+M+ genotype (dyslipidemia group: [6.54±0.58] vs [5.58±0.83] mmol/L, P<0.01; control group: [5.43±0.17] vs [4.39±0.62] mmol/L, P<0.01); the high density lipoprotein cholesterol (HDL-C) levels with X+X- genotype at XbaⅠ locus were significantly lower than those with X-X- genotype, whereas the low density lipoprotein cholesterol (LDL-C) levels were significantly higher than those with X-X- genotype (dyslipidemia group HDL-C: [1.08±0.27] vs [1.22±0.44] mmol/L, P=0.03; LDL-C: [3.88±0.63] vs [3.46±0.83] mmol/L, P=0.01; control group HDL-C: [1.31±0.43] vs [1.48±0.37] mmol/L, P=0.04; LDL-C: [3.19±0.54] vs [2.94±0.59] mmol/L, P=0.02); the HDL-C levels with E+E- genotype at EcoRⅠ locus were significantly lower than those with E+E+ genotype (dyslipidemia group: [1.01±0.18] vs [1.21±0.43] mmol/L, P=0.01; control group: [1.27±0.20] vs [1.47±0.40] mmol/L, P=0.03). The HDL-C levels in dyslipidemia group with X+X- genotype were significantly lower than the control group with X+X- genotype, but the LDL-C levels were significantly higher than the control group with X+X- genotype (HDL-C: [1.08±0.27] vs [1.31±0.43] mmol/L, P=0.01; LDL-C: [3.88±0.63] vs [3.19±0.54] mmol/L, P<0.01); the HDL-C levels in the dyslipidemia group with E+E- genotype were significantly lower than those in the control group ([1.01±0.18] vs [1.27±0.20] mmol/L, P<0.01). The multiple linear regression analysis showed that ApoB gene MspⅠ polymorphism was positively correlated with TC; XbaⅠpolymorphism was negatively correlated with HDL-C and positively correlated with LDL-C. Conclusion The ApoB gene MspⅠ and XbaⅠ polymorphisms may be associated with the levels of serum lipids. There is a tendency that the M+M- genotype can increase TC levels and the X+X- genotype can reduce HDL-C levels and increase LDL-C levels. |
| Key words: apolipoproteins B polymorphism lipids men |
