| 摘要: |
| 目的 探讨甲状腺良性结节射频消融后组织内热休克蛋白70(heat shock protein, HSP70)表达的变化及其潜在意义。方法 对50枚甲状腺良性实性结节实施超声引导下射频消融治疗,设定结节的中央区、边缘区和过渡区,于消融前后对应各穿刺取材1次,采用免疫组织化学染色法半定量测定其内HSP70的表达,对比消融前后及消融后不同区域HSP70的表达差异。结果 50枚甲状腺实性结节中央区和边缘区HSP70的表达在消融前、后无明显差异,过渡区HSP70的表达消融后强于消融前(P<0.05);消融治疗后HSP70的表达存在过渡区强于中央区和边缘区(P<0.05),而中央区与边缘区无明显差异。结论 热消融治疗过程中存在甲状腺结节周围过渡区HSP70高表达,这可能有助于保护正常腺体组织免受热损伤。 |
| 关键词: 射频消融 热休克蛋白70 甲状腺结节 免疫组织化学染色 |
| DOI：10.3724/SP.J.1008.2014.01215 |
| 投稿时间：2014-06-24修订日期：2014-10-09 |
| 基金项目:国家自然科学基金(81171436). |
|
| Effect of thermal ablation on HSP70 expression in benign thyroid nodules |
| YAN Lei1△,ZHANG Jian-quan1△*,PAN Qian1,ZHENG Jian-ming2,LÜ Yan-hong3 |
(1. Department of Ultrasound in Medicine, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;
2. Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;
3. Hospital of Yantai Military Garrison, Yantai 264000, Shandong, China
△Co-first authors.
*Corresponding authors) |
| Abstract: |
| Objective To explore the changes of HSP70 expression after radiofrequency ablation of benign thyroid nodules and to discuss the potential relevance. Methods Ultrasound-guided radiofrequency ablation (RFA) was conducted for treating 50 solid thyroid nodules in 50 patients. Three different regions of interest (ROI), namely the central region, marginal region and transitional region, were set on each nodule for quantitative investigation of HSP70 expression by using immunohistochemistry method. Each ROI experienced histological sampling by using core-needle biopsy before and after RFA. HSP70 expression was compared for the same ROI before and after ablation and between different ROIs after ablation. Results HSP70 expression had no significant changes in the central and marginal regions before and after ablation in the 50 solid thyroid nodules, and the expression was significantly enhanced in the transitional region after ablation (P<0.05). After ablation the expression of HSP70 in the transitional region was significantly higher than those in the central and marginal regions (P<0.05), with no significant difference found between the latter two regions. Conclusion Increased expression of HSP70 in the transitional region might protect normal glandular tissue against heat damage during thermal ablation treatment of benign thyroid nodules. |
| Key words: radiofrequency ablation heat shock protein 70 thyroid nodule immunohistochemistry |
