| 摘要: |
| 目的 观察比较低温冷冻后的富血小板血浆(PRP)在兔颅骨缺损修复中的效果。方法 将15只体质量为 2.5 kg 的健康新西兰雄性大白兔,沿兔颅顶中线制备3个骨缺损孔,分别植入－80℃低温冷冻自体PRP凝胶+羟基磷灰石/磷酸三钙复合体(HAP/TCP),(实验孔)、生理盐水+HAP/TCP(空白孔)、新鲜自体PRP凝胶+ HAP/TCP(对照孔),于术后4周、8周和12周时分别处死5只并通过大体观察、X线检查、组织学观察及CT值测量等方法比较低温冷冻PRP对兔颅骨缺损修复的影响。结果 术后4周、8周时,实验孔及对照孔成骨质量明显优于空白孔,两组CT值均较空白孔高[术后4周时实验孔、对照孔、空白孔分别为(376.8±50.41)、(414±71.41)、(94.42±37.02),术后8周时分别为(750.46±91.26)、(682.22±111.53)、(444.04±47.12)],差异均有统计学意义(P<0.01);而术后4周、8周时实验孔CT值与对照孔比较,差异均无统计学意义(P>0.05)。术后12周时,实验孔、对照孔和空白孔的成骨质量差异不明显,各孔间CT值差异均无统计学意义(P>0.05)。结论 PRP经低温冷冻保存后仍然和新鲜PRP一样,具有早期促进骨缺损修复的效果。 |
| 关键词: 低温保存 富血小板血浆 骨缺损 骨修复 |
| DOI：10.3724/SP.J.1008.2015.00391 |
| 投稿时间：2014-09-24修订日期：2015-01-12 |
| 基金项目:南京战区医药卫生科研基金[联卫[2008]533号(08MB124)]. |
|
| Cryopreserved platelet-rich plasma enhancing calvarial bone defect repair in rabbits: an observation of outcome |
| CHEN Jun1,ZHAO Yun-fu1*,ZHANG Qing-fu2*,LIU Guo-qin2,LIU Man-jiao3,YAO Tian2 |
(1. Department of Stomatology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;
2. PLA Diagnosis & Treatment Center of Oral Diseases, No.411 Hospital of PLA, Shanghai 200081, China;
3. Department of Clinical Laboratory, No.411 Hospital of PLA, Shanghai 200081, China
*Corresponding authors) |
| Abstract: |
| Objective To observe the outcome of cryopreserved platelet-rich plasma(PRP) in repairing rabbit calvarial bone defect. Methods Totally 15 male New Zealand rabbits, weighing 2.5 kg, were used in this study. Three defect holes were made along the calvarial midline in each rabbit; the upper defect holes were repaired with hydroxyapatite/tricalcium phosphate (HAP/TCP) compounded －80℃ cryopreserved PRP gel (experimental hole), the middle defect hole with HAP/TCP compounded physiological saline (blank hole), and the lower defect hole with HAP/TCP compounded fresh PRP gel (control hole). Five rabbits were sacrificed at 4, 8, and 12 weeks after operation, respectively. Gross observation, X-ray examination, histological observation, and CT scan were performed for the harvested samples, so as to observe whether －80℃ cryopreserved PRP gel could promote the defect repair. Results It was found that the osteogenesis of the defects in the experimental holes and the control holes were better than that in the blank holes at 4 weeks and 8 weeks after operation, with the CT values in the experimental hole, control hole and blank hole being (376.8±50.41), (414±71.41), and (94.42±37.02) at 4 weeks after operation and (750.46±91.26), (682.22±111.53), and (444.04±47.12) at 8 weeks after operation, respectively, showing significant differences(P<0.01); but there was no significant difference between the experimental holes and the control holes at both 4 weeks and 8 weeks after operation(P>0.05). At 12 weeks after operation, no differences in the osteogenesis of the defects were noticed between the three holes(P>0.05). Conclusion Cryopreserved PRP is confirmed to have the same effect as fresh PRP in enhancing early repair of calvarial bone defects. |
| Key words: cryopreservation platelet-rich plasma bone defect bone repair |
