| 摘要: |
| 目的 评价TiO2-xNx薄膜托槽的细胞生物相容性,并探讨掺氮含量与薄膜厚度对其生物相容性的影响。 方法 制备TiO2-xNx薄膜托槽试样,将L929细胞分成薄膜托槽组和对照组,其中薄膜托槽组分成不同氮含量组(氩气/氮气比分别为30:1、30:1.5、30:2)和不同厚度组(溅射时间分别为30、60、90 min)。通过对L929细胞进行细胞黏附实验、MTT实验和乳酸脱氢酶(LDH)释放实验评价TiO2-xNx薄膜托槽的细胞生物相容性。采用SPSS 19.0统计软件,通过单因素方差分析对实验数据进行统计学分析。 结果 L929细胞在薄膜托槽组和对照组托槽表面的黏附和伸展状态均良好。不同氮含量组(24、48、72 h)和厚度组(24、72 h)的细胞相对增殖率与对照组之间差异均有统计学意义(P<0.05),所有组别的相对增殖率均在80%以上。随着氮含量的增加,细胞相对增殖率降低;随着厚度的增加,细胞相对增殖率增加。LDH实验结果显示,随着氮含量的增加LDH活性增加,随着厚度的增加LDH活性降低,所有组别之间差异均无统计学意义(P>0.05)。 结论 TiO2-xNx薄膜托槽细胞毒性为0级,掺氮含量和薄膜厚度因素与L929细胞生物相容性有关。 |
| 关键词: 正畸托槽 TiO2-xNx 生物学效应 生物相容性 |
| DOI：10.3724/SP.J.1008.2015.1207 |
| 投稿时间：2015-03-20修订日期：2015-06-14 |
| 基金项目:甘肃省自然科学基金(1208RJZA236). |
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| In vitro biocompatibility of orthodontic brackets coated with TiO2-xNx thin film |
| FAN Ling-ying,ZHENG Cheng-dong,LIN Bing-peng,CAO Lin,CAO Bao-cheng* |
(Department of Orthodontics, School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu, China
*Corresponding author.) |
| Abstract: |
| Objective To evaluate the biocompatibility of orthodontic brackets coated with TiO2-xNx film, and to explore the effects of nitrogen-content and thickness of the film on the film biocompatibility. Methods We successfully prepared the TiO 2-xNx film brackets samples. L929 cells were divided into film brackets group and control group. The film brackets group was further divided into the nitrogen content groups (argon/nitrogen gas flow ratio of 30: 1, 30:1.5, and 30: 2) and thickness groups (sputtering time were 30, 60, and 90 min). L929 cells were used to evaluate the biocompatibility of TiO2-xNx film of orthodontic brackets by the methods of cell adhesion, MTT assay and the lactate dehydrogenase LDH release assay. The experimental data were analyzed by SPSS 19.0 software, and the results were analyzed by one-way ANOVA. Results The L929 cells adhered and spread well on the surface of the brackets both in the film brackets group and the control group. Although the cell proliferation rates of the nitrogen content groups at 24, 48, and 72 h, and of the thickness group at 24 and 72 h were significantly different from that of the control group (P<0.05), the relative proliferation rates were higher than 80% in all the groups. The cell proliferation rate decreased with the increase of nitrogen content, and increased with the increase of film thickness. According to the LDH assay, LDH activity increased with the increase of nitrogen content, and decreased with the increase of film thickness, but there was no significant difference among all the groups (P>0.05). Conclusion The cytotoxicity of TiO2-xNx film is at grade 0 level, and nitrogen content and film thickness are relevant factors of the biocompatibility of L929 cells. |
| Key words: orthodontic brackets TiO2-xNx biological effects biocompatibility |
