| 摘要: |
| 目的 探讨唾液淀粉酶(sAA)活性、流率和pH值以及sAA活性与流率相关性的性别差异。 方法 采集105名健康志愿者酸刺激前后的唾液标本,测定其sAA活性、流率和pH值,分析sAA活性和流率的相关性,比较唾液测定指标之间的性别差异。 结果 柠檬酸刺激能增加sAA活性、流率和pH值,其中以流率增加最大(P <0.01);sAA活性、流率和pH值在酸刺激前后以及对刺激的反应(前后的差值)都不存在性别差异;女性在酸刺激前后sAA活性和流率之间以及sAA活性差值和流率差值之间都存在正相关(P <0.05),并且相关系数相对稳定;男性只在酸刺激后的sAA活性和流率之间表现正相关(P <0.01)。 结论 sAA活性、流率和pH值在基础和应激状态下都没有性别差异,但是sAA活性和流率之间的相关性存在一定的性别差异。 |
| 关键词: 唾液 α淀粉酶 流率 pH值 柠檬酸 性别 |
| DOI：10.3724/SP.J.1008.2015.00883 |
| 投稿时间：2015-01-10修订日期：2015-03-24 |
| 基金项目:国家自然科学基金(81102703),广东省科技计划项目(2013A032500005),广东省中医药管理局项目(20123001),广东药学院基础学院教改项目. |
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| Sex differences of salivary α-amylase activity, flow rate and pH value before and after acid stimulation |
| YANG Ze-min1*,LIN Jing1,CHEN Long-hui2,ZHANG Min3,CHEN Wei-wen2,YANG Xiao-rong4 |
(1. Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong, China;
2. Pi-Wei Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong, China;
3. Department of Traditional Chinese Medicine, Maternal and Child Health Hospital of Haizhu District, Guangzhou 510240, Guangdong, China;
4. Department of Clinical Laboratory, the First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, Guangdong, China
*Corresponding author) |
| Abstract: |
| Objective To explore sex differences of salivary α-amylase activity (sAA), flow rate, pH value and correlation between sAA activity and flow rate. Methods Saliva samples were collected from 105 healthy volunteers before and after citric acid stimulation. Salivary sAA activity, flow rate and pH value were determined for each samples, and the correlation between sAA activity and flow rate was analyzed. The sex differences of the indices mentioned above were analyzed. Results Citric acid stimulation significantly increased salivary sAA activity, flow rate and pH value, with flow rate undergoing the greatest increase (P <0.01). No significant sex differences in sAA activity, flow rate or pH value were found at baseline, stimulation or acute responses (delta value) to citric acid stimulation. For female subjects, significant positive correlations were found between sAA activity and flow rate in unstimulated and stimulated saliva, between sAA activity delta value and flow rate delta value (P <0.05), and their coefficients kept relatively stable. For male subjects, significantly positive correlation was only found between stimulated sAA activity and flow rate (P <0.01). Conclusion Our study indicates that no sex difference in the sAA activity, flow rate or pH value at basal and acute responses to citric acid stimulation. However, certain sex difference is indicated in correlation between sAA activity and flow rate. |
| Key words: salivary α-amylase flow rate pH value citric acid sex |
