| 摘要: |
| 目的 建立同时测定金龙蛇口服液中5种主要有效成分的HPLC法。 方法 色谱柱为SunFireTM C18柱(4.6 mm×250 mm,5 μm),以乙腈(B)-0.2%甲酸(A)为流动相进行梯度洗脱(0~10 min,10%~19% B;10~32 min,19% B),流速为1.0 mL/min,柱温40℃,进样量10 μL,检测波长为285 nm。 结果 柚皮苷、橙皮苷、新橙皮苷、麦角甾苷和甘草苷的质量浓度分别在2.00~60.00、2.12~63.60、1.68~50.40、2.00~60.00和2.00~60.00 μg/mL范围内呈良好的线性关系(r≥0.999 9)。5种成分的日内精密度和日间精密度RSD分别小于2.30%和3.82%,24 h内稳定性RSD<4.47%;平均加样回收率分别为95.11%、93.73%、96.39%、98.02% 和95.12%;RSD分别为2.08%、4.05%、4.02%、6.01%和4.56%。 结论 该方法简便、准确、灵敏度高、重现性好,适用于金龙蛇口服液的质量控制和评价。 |
| 关键词: 金龙蛇口服液 柚皮苷 橙皮苷 新橙皮苷 麦角甾苷 甘草苷 高压液相色谱法 |
| DOI：10.3724/SP.J.1008.2015.00917 |
| 投稿时间：2015-01-12修订日期：2015-03-12 |
| 基金项目: |
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| HPLC for simultaneous determination of naringin, hesperidin, neohesperidin, acteoside and liquiritin in Jinlongshe oral solution |
| WANG Qing-hua1,2,GAO Shou-hong1,XIONG Xiao-juan2,LU Wen-quan1,PIAO Shu-juan1,ZHANG Feng1* |
(1. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;
2. Bioengineering Research Institute of Yichun University, Yichun 336000, Jiangxi, China
*Corresponding author) |
| Abstract: |
| Objective To establish an HPLC method for simultaneous determination of five effective components (naringin, hesperidin, neohesperidin, acteoside and liquiritin) in the Jinlongshe oral solution. Methods SunFireTM C18 (4.6 mm×250 mm, 5 μm) column was used. The mobile phase was acetonitrile (B) and 0.2% formic acid (A), with gradient elution program as follow: 0-10 min, 10%-19% B; 10-32 min, 19% B. The flow rate was 1.0 mL/min, and the temperature was at 40℃. The injection volume was 10 μL, and the detection wavelength was set at 285 nm. Results There were good linearities for concentrations of naringin, hesperidin, neohesperidin, acteoside and liquiritin within the range of 2.00-60.00, 2.12-63.60, 1.68-50.40, 2.00-60.00 and 2.00-60.00 μg/mL (r≥0.999 9), respectively. RSD of the intra-day and inter-day precisions of the five components were less than 2.30% and 3.82%, respectively. RSD of the stability in 24 h was less than 4.47%. The average recoveries were 95.11%, 93.73%, 96.39%, 98.02% and 95.12%, with the RSD being 2.08%, 4.05%, 4.02%, 6.01% and 4.56%, respectively. Conclusion The present method has been proven to be convenient, accurate, sensitive and with good reproducibility, and can be applied for the quality control and assessment of Jinlongshe oral solution. |
| Key words: Jinlongshe oral solution naringin hesperidin neohesperidin acteoside liquiritin high pressure liquid chromatography |
