| 摘要: |
| 目的 探讨雪旺细胞与细菌纤维素体外共培养时的生物相容性,为细菌纤维素制备成组织工程化神经提供实验依据。方法 将背根神经节与细菌纤维素膜体外共培养,分别在培养的第1、2、5天通过扫描电镜和光镜观察雪旺细胞的形态及增殖、迁移情况,免疫细胞化学染色检测S-100蛋白表达情况;将坐骨神经来源的纯化雪旺细胞在细菌纤维素浸出液中培养,分别在24、48、72 h采用倒置显微镜和免疫细胞化学染色观察雪旺细胞形态及S-100蛋白的表达情况,并通过MTT检测细胞活力,流式细胞仪检测细胞周期来综合评价雪旺细胞与细菌纤维素的生物相容性。结果 雪旺细胞能在细菌纤维素膜表面正常生长、迁移;与普通培养基相比,细菌纤维素浸出液对雪旺细胞的生长、增殖及S-100蛋白的表达无明显影响。结论 雪旺细胞能够在细菌纤维素膜表面及细菌纤维素浸出液中良好生长,体外与细菌纤维素具有较好的生物相容性。 |
| 关键词: 背根神经节 坐骨神经 细菌纤维素 生物相容性 雪旺细胞 细胞周期 |
| DOI：10.3724/SP.J.1008.2015.00324 |
| 投稿时间：2014-11-29修订日期：2015-01-09 |
| 基金项目:第二军医大学长海医院"1255 "学科建设计划 (CH125530900). |
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| In vitro biocompatibility of bacterial cellulose and Schwann cells |
| LIU Wei1,GU Wei-yong2,ZHOU Xia-jun1,ZHANG Tian-yi3* |
(1. Department of Neurology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;
2. Department of Pathology, the Obstetrics and Gynecology Hospital Affiliated to Fudan University, Shanghai 200090, China;
3. Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, Jiangsu, China
*Corresponding author) |
| Abstract: |
| Objective To observe the biocompatibility of bacterial cellulose and Schwann cells during in vitro co-culture, so as to lay a foundation for preparing tissue-engineered nerve with bacterial cellulose. Methods The bacterial cellulose was co-cultured with the dorsal root ganglion. The morphology, proliferation, and migration of the Schwann cells were examined by scanning electron microscope and optical microscope at day 1, 2, and 5, and the protein expression of S-100 was detected by immunocytochemical method. Schwann cells were also cultivated in the leach liquor of bacterial cellulose, and then optical microscope and immunocytochemical were used to observe the morphology and S-100 protein expression after 24, 48 and 72 hours; MTT and flow cytometry were used to evaluate the biocompatibility of the bacterial cellulose and Schwann cells. Results Schwann cells could grow and migrate on the surface of bacterial cellulose's membrane. The Schwann cell growth, proliferation and S-100 expression were not significantly affected by the leach liquor of bacterial cellulose as compared with the control group. Conclusion Schwann cells can grow well on the surface of the bacterial cellulose membrane and in its leach liquor, suggesting good biocompatibility with bacterial cellulose in vitro. |
| Key words: dorsal root ganglia sciatic nerve bacterial cellulose biocompatibility Schwann cells cell cycle |
