| 摘要: |
| 目的 观察水飞蓟素对心肌梗死小鼠的心肌保护作用及可能机制。 方法 将60只雄性小鼠随机分为假手术组、急性心肌梗死组、急性心肌梗死+水飞蓟素组和急性心肌梗死+溶剂组,每组15只。造模术后第2天开始给药,持续4周。建模成功4周后进行血流动力学检测和心脏超声检查评价心脏功能,H-E染色评价心肌梗死程度,TUNEL观察细胞凋亡情况并计算凋亡指数,蛋白质印迹法检测凋亡蛋白Bcl-2、Bax和Cleaved Caspase-3的表达。 结果 与急性心肌梗死组小鼠相比,水飞蓟素可改善急性心肌梗死小鼠心室收缩功能和血流动力学指标, 减小心肌梗死面积、减轻梗死区组织病理学改变(P<0.05),降低心肌细胞凋亡指数(P<0.05),增强 Bcl-2蛋白表达并减弱Bax和Cleaved Caspase-3蛋白表达(P<0.05)。 结论 水飞蓟素可减轻小鼠心肌梗死,改善心肌梗死小鼠心功能,其机制与减少心肌细胞的凋亡有关。 |
| 关键词: 水飞蓟素 血流动力学 心肌收缩 心肌梗死 细胞凋亡 |
| DOI：10.3724/SP.J.1008.2015.01309 |
| 投稿时间：2015-04-02修订日期：2015-08-14 |
| 基金项目:上海市科委重点基础项目(10411963900). |
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| Silymarin alleviates myocardial infarction by inhibiting myocardial cell apoptosis |
| CHEN Jia1,CAO Zhi-yong1,HE Yong-hui2,WU Zong-gui1,REN Yu-sheng1* |
(1. Department of Cardiology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;
2. Department of Urology, People's Hospital Affiliated to Jiangsu University, Zhenjiang 212002, Jiangsu, China
*Corresponding author.) |
| Abstract: |
| Objective To evaluate the cardioprotective effects of silymarin on mice with acute myocardial infarction (AMI) and its possible mechanism. Methods A total of 60 male C57BL/6 mice were randomly divided into 4 groups: Sham group, AMI group, AMI+Silymarin group, and AMI+Vehicle group. Drug administration was started at the second day after modeling and lasted for four weeks. Four weeks after modeling, hemodynamic parameters and quantitative echocardiographic assessments were obtained to evaluate the cardiac function. Myocardium infarct area was estimated by H-E staining. Cell apoptosis was observed by TUNEL and apoptotic index was calculated. Protein expressions of Bcl-2, Bax and Cleaved Caspase-3 were detected by Western blotting analysis. Results Compared with AMI group, AMI+Silymarin group had improved hemodynamic parameters and cardiac function, significantly reduced infarction area and histopathology changes of the infarcted area (P<0.05), significantly decreased cardiomyocyte apoptotic index (P<0.05), significantly increased protein expression of Bcl-2 and significantly decreased expression of Bax and Cleaved Caspase-3 (P<0.05). Conclusion Silymarin can reduce infarction area and improve cardiac function in mice, which might be related to inhibition of the myocardial apoptosis. |
| Key words: silymarin hemodynamics myocardial contraction myocardial infarction apoptosis |
