| 摘要: |
| 目的 阐明运动锻炼对雌激素缺乏所致小鼠抑郁样行为的影响及其炎症机制, 并为运动锻炼治疗更年期抑郁症提供理论依据。方法 将60只雌性小鼠随机分为假手术组(Sham组)、假手术+运动组(Sham Ex组)、去卵巢组(Ovx组)、去卵巢 运动组(Ovx Ex组)。建立假手术和去卵巢模型, 术后1周, 运动组进行为期5周的跑台锻炼。运用糖水实验、旷场实验和悬尾实验来观察各组小鼠的行为学改变情况。提取各组小鼠海马组织, 用ELISA试剂盒检测5-羟色胺(5-hydroxytryptamine,5-HT)、吲哚胺2, 3-双加氧酶(indoleamine 2, 3-dioxygenase, IDO)及炎性细胞因子的水平。结果 与Sham组比较, Ovx组小鼠发生了抑郁样行为改变, 包括兴趣缺失、探究性行为减少、绝望情绪增加;运动锻炼可以改善Ovx小鼠抑郁样行为。与Sham组比较, Ovx组小鼠海马中5-HT含量降低 (P<0.01), IDO含量升高 (P<0.05);给予Ovx小鼠运动锻炼能够逆转上述改变。与Sham组相比, Ovx组小鼠海马中促炎细胞因子IL-1β (P<0.05)、IL-6 (P<0.01)和IFN-γ (P<0.01)含量增加, 抑炎细胞因子IL-4 (P<0.01)、IL-10 (P<0.05)含量降低;给予Ovx小鼠运动锻炼能够逆转IL-1β、IFN-γ 和IL-4表达的改变。结论 运动锻炼可能通过减轻海马炎症反应来改善雌激素缺乏所致抑郁样行为。 |
| 关键词: 运动 抑郁 雌激素类 5-羟色胺 吲哚胺2,3-双加氧酶 细胞因子 |
| DOI：10.3724/SP.J.1008.2015.00477 |
| 投稿时间：2014-12-19修订日期：2015-01-29 |
| 基金项目:上海市自然科学基金(13ZR1448700). |
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| Effect of exercise on estrogen deficiency-induced depression-like behavior and its inflammatory mechanisms |
| BAO Qing-yue1,WANG Yu-jun2,XU Yong-jun1,SHENG Hui1,CAO Min-jia3,NI Xin1* |
(1. Department of Physiology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China;
2. School of Kinesiology, Shanghai University of Sport, Shanghai 200438, China;
3. College of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China
*Corresponding author) |
| Abstract: |
| Objective To explore whether exercise training can ameliorate estrogen deficiency-induced depression-like behavior and its mechanisms, so as to provide evidence for treating menopausal depression with exercise. Methods A total of 60 female adult C57BL/6J mice were randomly assigned to Sham-sedentary (Sham), Sham-exercise (Sham+Ex), Ovx-sedentary (Ovx) and Ovx-exercise (Ovx Ex) groups. Sham operation or bilateral Ovx was performed. After recovery for 1 week, Sham Ex and Ovx Ex mice were adapted to the treadmill for 5 weeks. We use sucrose consumption test, open field test and tail suspension test to inspect the mice of behavior change; then the mice were decapitated, and the serum and hippocampus samples were collected. 5-hydroxytryptamine (5-HT), indoleamine 2,3-dioxygenase (IDO) and inflammatory cytokine in the hippocampus were determined using a commercial ELISA kit. Results Compared with Sham mice, Ovx mice developed depression-like behaviors, including loss of interests, decreased exploratory behavior and increased behavioral despair, and exercise training could ameliorate these depression-like behaviors. Compared with the Sham group, Ovx group had significantly decreased hippocampal 5-HT level (P<0.01) and significantly increased IDO level (P<0.05) in the hippocampus, which were reversed by exercise training. The hippocampal levels of proinflammatory cytokines, IL-1β (P<0.05), IL-6 (P<0.01) and IFN-γ (P<0.01) in Ovx group were significantly increased compared to Sham mice, while the anti-inflammatory cytokines, IL-4 (P<0.01) and IL-10 (P<0.05), were significantly decreased in Ovx mice; exercise training could also reverse the alterations of IL-1β, IFN-γ and IL-4 levels in Ovx mice. Conclusion Exercise training can ameliorate estrogen deficiency-induced depression-like behaviors, which may be through improving inflammation in the hippocampus. |
| Key words: exercise depression estrogens 5-hydroxytryptamine indoleamine 2,3-dioxygenase cytokines |
