| 摘要: |
| 目的 探讨快速眼球运动(rapid eye movement,REM)睡眠剥夺后大鼠下丘脑背内侧核神经肽S (neuropeptide S,NPS)表达的变化。方法 24只大鼠随机均分为正常对照(CC)组、环境对照(TC)组、REM睡眠剥夺(SD)组,采用改良多平台睡眠剥夺法建立大鼠REM睡眠剥夺模型,运用免疫组织化学分析法及原位杂交法观察大鼠经3 d REM睡眠剥夺后下丘脑内NPS蛋白及mRNA的表达变化。结果 NPS蛋白及mRNA的表达在CC组和TC组没有显著差异。REM睡眠剥夺后,大鼠下丘脑背内侧核内的NPS蛋白及mRNA表达上调, SD组下丘脑背内侧核内NPS蛋白及mRNA阳性细胞数分别为(27.86±2.47)和(25.75±2.12),较CC组(16.75±2.12和19.63±1.85)、TC组(18.60±1.60和18.50±1.69)升高(P<0.05)。结论 REM睡眠剥夺后下丘脑内觉醒相关区域NPS表达增加,说明当REM睡眠减少时机体会主动调节NPS的分泌,提示NPS与睡眠觉醒的调节机制有关。 |
| 关键词: REM深眠状态 睡眠剥夺 下丘脑背内侧核 神经肽S |
| DOI：10.3724/SP.J.1008.2015.00320 |
| 投稿时间：2015-01-22修订日期：2015-03-02 |
| 基金项目:国家自然科学基金 (81100991,81171252,81200061). |
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| Effect of rapid eye movement sleep deprivation on expression of neuropeptide S in dorsomedial nucleus of rat hypothalamus |
| ZHAO Fei,ZHAO Zheng-qing,LI Yan-peng,ZHUANG Jian-hua,ZHAO Zhong-xin* |
(Department of Neurology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
*Corresponding author) |
| Abstract: |
| Objective To observe the changes of the neuropeptide S (NPS) expression in dorsomedial nucleus of rat hypothalamus after rapid eye movement (REM) sleep deprivation. Methods Totally 24 adult male rats were evenly randomized into 3 groups: normal control group (CC), tank control group (TC) and REM sleep deprivation group (SD). Modified multiple platform method (MMPM) was used to establish REM sleep deprivation model in rats. Immunohistochemistry and hybridization in situ were applied to examine the expression of NPS protein and mRNA in the rat hypothalamus. Results There was no significant differences in the expression of NPS protein or mRNA in rats hypothalamus between CC group and TC group. After REM sleep deprivation, NPS protein and mRNA positive cells in rats hypothalamus in SD group were (27.86±2.47) and (25.75±2.12), respectively, which were significantly more than those in CC group and TC group (16.75±2.12 and 19.63±1.85 in CC group, 18.60±1.60 and 18.50±1.69 in TC group; P<0.05). Conclusion The expression of NPS is increased in the awakening region of rat hypothalamus after REM sleep deviation, suggesting that NPS might be related to the sleep awakening mechanism. |
| Key words: REM sleep parasomnias sleep deprivation dorsomedial hypothalamic nucleus neuropeptide S |
