| 摘要: |
| 目的 观察β-arrestin2在卵巢切除(OVX)大鼠心脏的表达变化,探索β-arrestin2在雌激素改善OVX大鼠心血管功能中的作用。方法 以OVX手术绝经的大鼠为模型,通过监测血中雌激素水平以及子宫质量与体质量比的变化明确OVX模型成功。雌激素替代治疗4周,通过检测心脏质量与体质量比、血压和心率反映心血管功能情况,蛋白质免疫印迹检测心脏β-arrestin1、β-arrestin2和血管紧张素受体(AT1R)蛋白表达水平。结果 OVX组较假手术组血中雌激素水平下降(P<0.05)、子宫萎缩(P<0.05),血压升高(P<0.05),心率加快(P<0.05),心脏体质量比升高(P<0.05);雌激素替代治疗后,血压下降、心率减慢、心脏体质量比降低(P<0.05)。OVX组较假手术组心脏中β-arrestin2表达降低(P<0.05),雌激素可上调其表达,而β-arrestin1和AT1R表达水平无明显变化。结论 β-arrestin2在 OVX大鼠心脏中表达减少,而雌激素替代治疗可增加β-arrestin2在OVX大鼠心脏组织中的表达。 |
| 关键词: β-arrestin 1型血管紧张素受体 雌激素类 卵巢切除术 心血管功能 |
| DOI：10.3724/SP.J.1008.2015.00634 |
| 投稿时间：2015-03-06修订日期：2015-04-28 |
| 基金项目:国家自然科学基金 (81370363,31100831),上海市科委面上项目 (81270335). |
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| Estrogen replacement increases β-arrestin2 expression in cardiac tissues in ovariectomized rats |
| HAO Fan1△,TAN Xing2△,WANG Wei-zhong2,XU Ming-juan1* |
(1. Department of Obstetrics and Gynecology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;
2. Department of Physiology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China
△Co-first authors.
*Corresponding authors) |
| Abstract: |
| Objective To observe the expression of β-arrestin2 in cardiac tissues of ovariectomzied (OVX) rats, and to explore the role of β-arrestin2 in estrogen-mediated cardiovascular protection in OVX rats. Methods OVX rats were evaluated by monitoring the serum levels of estrogen and the ratio of uterine weight to body weight. The cardiovascular function of rats was assessed by measuring the ratio of heart weight to body weight, blood pressure and heart rate after estrogen replacement treatment for 4 weeks, Western blotting analysis was performed to detect the protein expression of β-arrestin1, β-arrestin2 and angiotensin receptor type 1 (AT1R) in the cardiac tissues. Results Compared with rats in the sham group, the serum estrogen level and the ratio of uterine weight to body weight in OVX rats were decreased significantly (P<0.05); meanwhile, the cardiovascular parameters including blood pressure, heart rate and the ratio of heart weight to body weight were significantly increased (P<0.05) in OVX rats, and they were attenuated after treatment with estrogen replacement. Compared with the sham controls, the cardiac expression of β-arrestin2 was decreased significantly in OVX rats (P<0.05), with up-regulated estrogen expression and unchanged expression of β-arrestin1 and AT1R. Conclusion β-Arrestin2 expression is decreased in the cardiac tissues of OVX rats, which can be increased by estrogen replacement therapy. |
| Key words: β-arrestin type 1 angiotensin receptor estrogens ovariectomy cardiovascular function |
