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| miR-141抑制头颈部鳞癌细胞增殖 |
| 王国栋1Δ,黄建涛1Δ,赵云富1,刘渊1,陈潇卿1,吴洋1*,汪大林2* |
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(1. 第二军医大学长征医院口腔科, 上海 200003;
2. 第二军医大学长海医院口腔科, 上海 200433
△共同第一作者
*通信作者) |
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| 摘要: |
| 目的 考察microRNA-141(miR-141)对人头颈部鳞癌细胞增殖的影响。 方法 通过qRT-PCR分析19例头颈部鳞状细胞癌患者的肿瘤组织与癌旁正常组织标本中miR-141的表达。通过miR-141 mimics 或miR-141 反义寡核苷酸(ASO)上调或抑制人喉癌细胞株(Hep-2)和人舌鳞状细胞癌细胞株(SCC-9)细胞中miR-141的表达水平。采用MTT实验分析上调或抑制miR-141的表达水平对Hep-2和SCC-9细胞增殖的影响。利用生物信息学方法预测miR-141的靶基因。 结果 qRT-PCR的结果表明,头颈部鳞状细胞癌组织的miR-141的表达水平低于癌旁正常组织(P<0.05);上调miR-141的表达能够抑制Hep-2和SCC-9细胞的增殖(P<0.05),而抑制miR-141表达可以促进Hep-2和SCC-9细胞的增殖(P<0.05)。生物信息学方法预测miR-141的靶基因可能为ZEB1基因。 结论 miR-141可能通过ZEB1基因抑制头颈部鳞状细胞癌的增殖。 |
| 关键词: microRNA-141 头颈部肿瘤 鳞状细胞癌 ZEB1 细胞增殖 |
| DOI：10.3724/SP.J.1008.2015.01314 |
| 投稿时间：2015-03-12修订日期：2015-10-02 |
| 基金项目:全军医学科技"十二五"科研项目(CWS11J300),上海市科委产学研医合作项目(12DZ1940503,13DZ1942704),军队卫生训练课题(2011XL015). |
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| miR-141 inhibits the proliferation of head and neck squamous cell carcinoma |
| WANG Guo-dong1Δ,HUANG Jian-tao1Δ,ZHAO Yun-fu1,LIU Yuan1,CHEN Xiao-qing1,WU Yang1*,WANG Da-lin2* |
(1. Department of Stomatology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;
2. Department of Stomatology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
△Co-first authors.
*Corresponding authors.) |
| Abstract: |
| Objective To study the effect of miR-141 on the proliferation of head and neck squamous cell carcinoma(HNSCC)cells. Methods The expressions of miR-141 in 19 HNSCC tissues and the matched adjacent normal tissues were analyzed by qRT-PCR. The miR-141 in Hep-2 or SCC-9 cells was overexpressed or down-regulated by miR-141 mimics or miR-141 ASO transfection. Then the effects of overexpression or down-regulation of miR-141 on cellular proliferation were analyzed by MTT experiment. The targeted genes of miR-141 were predicted by bioinformatics algorithms. Results The expression of miR-141 in HNSCC tissues was found significantly lower than that in matched normal tissue(P<0.05). Overexpression of miR-141 significantly inhibited the proliferation of Hep-2 and SCC-9 cells, and down-regulation of miR-141 significantly promoted the proliferation of Hep-2 and SCC-9 cells(P<0.05). Bioinformatics algorithms showed that ZEB1 was likely to be a targeted gene of miR-141 in HNSCC. Conclusion miR-141 may inhibit HNSCC cell proliferation via ZEB1. |
| Key words: microRNA-141 head and neck neoplasms squamous cell carcinoma ZEB1 cell proliferation |
