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| 实验室检测埃博拉病毒对诊疗的临床意义 |
| 胡宗海1,2,刘毅1,3,谢静2,邹自英2,彭燕2,刘媛2,吴丽娟2* |
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(1. 中国人民解放军第二批援利医疗队;
2. 成都军区总医院实验医学中心微生物免疫科, 高湿医学全军重点实验室, 成都 610083;
3. 第二军医大学长海医院麻醉科, 上海 200433
*通信作者) |
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| 摘要: |
| 目的 分析实验室PCR检测结果对埃博拉病毒病患者诊疗的临床意义。方法 在2014年11月14日至2015年3月14日期间,利比里亚中国埃博拉治疗中心(Ebola Treatment Unit,ETU)共收治住院患者101例,送检172份标本,通过实验室实时荧光PCR检测病毒核酸及CT值,分析实验室检测结果与病情变化及预后的关系。结果 共有10例埃博拉病毒核酸阳性患者,死亡4例,治愈6例。4例死亡病例中,病毒核酸PCR CT值均较低,表明其体内病毒载量较高;在6例治愈病例中,随着病情的好转,病毒核酸PCR CT值逐渐升高,表明病毒载量逐渐降低。结论 实验室实时荧光PCR检测病毒核酸及其CT值可为临床诊断、治疗提供指导。 |
| 关键词: 埃博拉病毒 埃博拉病毒病 聚合酶链反应 诊断 治疗 |
| DOI：10.3724/SP.J.1008.2015.00598 |
| 投稿时间：2015-04-02修订日期：2015-05-20 |
| 基金项目: |
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| Laboratory tests for clinical diagnosis and treatment of Ebola virus disease |
| HU Zong-hai1,2,LIU Yi1,3,XIE Jing2,ZOU Zi-ying2,PENG Yan2,LIU Yuan2,WU Li-juan2* |
(1. The Second Medical Team of the Chinese People's Liberation Army to Liberia;
2. Department of Microbiology and Immunology, Center of Laboratory Medicine, Key Laboratory of High Humidity Medicine of PLA, Chengdu General Hospital, PLA Chengdu Military Area Command, Chengdu 610083, Sichuan, China;
3. Department of Anesthesiology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
*Corresponding authors) |
| Abstract: |
| Objective To analyze the clinical significance of PCR results for diagnosis and treatment of Ebola virus disease. Methods From 2014 November to 2015 March, a total of 101 patients were admitted to China Ebola Treatment Unit (ETU) in Liberia, and 172 specimens were sent for laboratory test: detection of Ebola virus RNA using real time PCR. The result of detection was recorded as positive or negative. If positive, the cycle threshold (CT) in real-time PCR was also reported. The relationship of laboratory findings with illness and prognosis was analyzed. Results Ten patients were identified as virus positive. The results of the first detection were positive in 8 patients and negative in 2 patients, and the 2 patients were positive in a second detection performed again 48 h later. The low CT values of PCR test observed in the 4 patients suggested high virus loads, and they died finally. In the 6 survived patients the CT values increased as their condition became better, which indicated that the virus loads decreased gradually. Conclusion Detection of Ebola virus mRNA by real-time PCR and the CT value can provide important reference for diagnosis and treatment of Ebola virus disease. |
| Key words: Ebola virus Ebola virus disease polymerase chain reaction diagnosis therapy |
