| 摘要: |
| 目的 采用自体移植方法建立子宫内膜异位症大鼠模型,比较不同移植部位的建模效果。方法 取40只雌性、成熟未交配SD大鼠,将自体子宫内膜组织分别移植于卵巢、宫骶韧带及腹壁上,术后28 d手术取出移植物,测量移植物的体积和质量,对移植物进行组织形态学观察并比较不同部位的成模率。结果 卵巢部位移植物体积和质量大于其他两个部位(P<0.05),而宫骶韧带及腹壁部位的移植物体积及质量差异无统计学意义。3个不同部位的成模率差异无统计学意义,且移植物组织病理学相似。结论 卵巢、宫骶韧带及腹壁3个部位的自体子宫移植均可建立子宫内膜异位症模型,病理改变与子宫内膜异位症患者相似,但卵巢部位建模效果最佳,更有利于子宫内膜异位症新的治疗方法的开发及研究。 |
| 关键词: 子宫内膜异位症 动物模型 大鼠 自体移植 |
| DOI：10.16781/j.0258-879x.2016.04.0513 |
| 投稿时间：2015-12-18修订日期：2016-03-31 |
| 基金项目: |
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| Comparison of autotransplantation sites in endometriosis rat model |
| HE Yan△,ZENG Jin-feng△,LIU Yu-huan* |
(Department of Gynecology & Obstetrics, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
△Co-first authors
*Corresponding author) |
| Abstract: |
| Objective To establish an endometriosis rat model by autotransplanting the endometrium in three different sites of the same rat, and to compare the effects of modeling. Methods The endometrium tissues of 40 adult unmated female SD rats were autotransplanted into the ovary, the uterosacral ligament, and the abdominal wall. Transplants were collected for volume and weight determination and pathological examination on the 28th day after surgery. The successful modeling rates of transplanting endometrium tissues to three different sites were compared. Results The volume and weight of transplants in the ovarian were greater than those in the other two sites (P<0.05), and the transplants on uterosacral ligament and abdominal wall had no notable difference in the volume or weight. The successful modeling rates of the three sites had no significant difference, and the transplants had similar histopathology. Conclusion Autotransplantation of endometriosis in the ovary, the uterosacral ligament, and the abdominal wall of rats can all establish the endometriosis model, and the pathological changes are similar to endometriosis patients; the best modeling effect is seen in the ovary site, which is more conducive to new treatments for endometriosis. |
| Key words: endometriosis animal models rats autologous transplantation |
