| 摘要: |
| 目的 观察不同年龄段大鼠腹膜结构的改变,寻找腹膜增龄性衰老的分子病理特点。方法 18只雄性SD大鼠随机分为3组:幼年组(1个月龄)、成年组(8个月龄)、老年组(18个月龄),每组6只;取壁层腹膜行H-E染色及β-半乳糖苷酶染色,观察大鼠腹膜形态改变。半定量计算腹膜厚度、腹膜间皮细胞数及单位面积腹膜血管数;用RT-PCR及蛋白质免疫印迹法观察脏层腹膜转化生长因子β1(transforming growth factor-beta 1, TGF-β1)、锌指蛋白转录因子15(Krüppel like factor 15,KLF15) mRNA和蛋白的表达情况。结果 随着年龄增加,大鼠腹膜增厚,腹膜间皮细胞减少,间皮下基质血管增生明显(P<0.05)。脏层腹膜TGF-β1表达逐渐上调(P<0.05),KLF15表达逐渐减少(P<0.05)。TGF-β1表达与KLF15表达呈负相关。结论 推测TGF-β1可能在大鼠腹膜增龄性衰老过程中起到促进作用,而KLF15因子可能抑制腹膜衰老。 |
| 关键词: 腹膜 衰老 转化生长因子β1 Krüppel样转录因子类 |
| DOI：10.16781/j.0258-879x.2016.04.0458 |
| 投稿时间：2016-01-12修订日期：2016-03-19 |
| 基金项目:福建省自然科学基金(2013J01340). |
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| Age-related changes of rat peritoneum tissue: molecular and pathology characteristics |
| LIN Qin1,2*,JIANG Xiao-juan1,LIU Li-li1 |
(1. The First Affiliated Clinical College, Fujian Medical University, Fuzhou 350005, Fujian, China;
2. Hypertension Research Institute of Fujian Province, Fuzhou 350005, Fujian, China
*Corresponding author) |
| Abstract: |
| Objective To observe the morphologic changes of rat peritoneum tissue in different age groups, so as to explore the age-related molecular and pathology characteristics. Methods Eighteen male SD rats were evenly randomized into three groups:1-month group (the juvenile group); 8-month group (the youth group); and 18-month group (the elderly group). The morphological changes of parietal peritoneum were observed by Hematoxylin-Eosin and β-gal staining. The peritoneal thickness, peritoneal mesothelial cells and blood vessel count under unit area were analyzed semi-quantitatively. The expressions of transforming growth factor-beta 1(TGF-β1) and krüppel like factor 15(KLF15) mRNA or protein in the rat mesenteric tissues were evaluated by RT-PCR and Western blotting analysis. Results The peritoneum gradually thickened with the increase of age, accompanied by reduced peritoneal mesothelial cells and increased interstitial vascular proliferation (P<0.05). The expression of TGF-β1 was gradually increased (P<0.05) and that of KLF15 was decreased (P<0.05) at mRNA and protein levels, and the expression of TGF-β1 was negatively associated with KLF15. Conclusion It is speculated that TGF-β1 may play a role in promoting the peritoneal aging process, while the KLF15 factor may inhibit the peritoneal senescence. |
| Key words: peritoneum aging transforming growth factor β1 Krüppel-like transcription factors |
