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骨关节炎关节液促进滑膜间充质干细胞软骨分化
崔岩1,张治宇1*,程战2,曾雪艳3,倪胜辉1
0
(1. 中国医科大学附属第四医院骨科, 沈阳 110032;
2. 解放军93277部队卫生队, 沈阳 110032;
3. 解放军463医院血液净化中心, 沈阳 110042
*通信作者)
摘要:
目的 观察骨关节炎(OA)关节液对滑膜间充质干细胞(SDSCs)软骨分化能力的影响。方法 用OA患者手术切除的滑膜组织标本分离培养SDSCs并诱导成软骨分化。将OA患者的关节液加入诱导后的SDSCs培养液中,模拟体内的OA关节环境继续培养(实验组),以不加OA关节液的SDSCs作为对照组。测量两组SDSCs形成的软骨小球的直径,采用实时荧光定量PCR和蛋白质印迹法检测成软骨相关标志物COL Ⅱ、Aggrecan、SOX9的mRNA及蛋白表达。结果 OA关节液处理16 d后,实验组软骨小球直径大于对照组(P<0.05),COL Ⅱ、Aggrecan、SOX9 mRNA和蛋白的表达均高于对照组(P<0.05)。结论 OA关节液能够促进SDSCs软骨分化。
关键词:  骨关节炎  关节液  间充质干细胞  软骨分化
DOI:10.16781/j.0258-879x.2016.06.0776
投稿时间:2016-02-12修订日期:2016-05-17
基金项目:
Synovial fluid of osteoarthritis promotes the differentiation of synovial mesenchymal stem cells
CUI Yan1,ZHANG Zhi-yu1*,CHENG Zhan2,ZENG Xue-yan3,NI Sheng-hui1
(1. Department of Orthopedics, the Fourth Affiliated Hospital of China Medical University, Shenyang 110032, Liaoning, China;
2. Medical Team, No. 93277 Troop of PLA, Shenyang 110032, Liaoning, China;
3. Blood Purification Center, No. 463 Hospital of PLA, Shenyang 110042, Liaoning, China
*Corresponding author)
Abstract:
Objective To explore the effect of synovial fluid of osteoarthritis (OA) on the differentiation of synovium-derived stem cells (SDSCs). Methods The SDSCs from the synovium of OA patients were identified and were induced to cartilage differentiation. The SDSCs were cultured with OA joint fluid (experimental group) or without OA joint fluid (control group). The diameter of the cartilage was measured. The mRNA and protein expressions of COL Ⅱ, Aggrecan and SOX9 were determined by RT-PCR and Western blotting analysis. Results The diameter of the cartilage in the experimental group was greater than that in the control 16 days after cultured with OA joint fluid (P<0.05). And the levels of COLⅡ, Aggrecan and SOX9 mRNA and protein in the experimental group was significantly higher than those in the control group (P<0.05). Conclusion The OA joint fluid can promote differentiation of SDSCs.
Key words:  osteoarthritis  synovial fluid  mesenchymal stem cells  cartilage differentiation