| 摘要: |
| 目的 建立一种开放性颅脑外伤海水浸泡动物模型，研究其伤情特点。方法 健康雄性SPF级SD大鼠100只，随机分为5组，分别为对照组、轻型颅脑外伤海水浸泡组、中型颅脑外伤海水浸泡组、重型颅脑外伤海水浸泡组和重型颅脑外伤生理盐水浸泡组。采用控制性皮质撞击法造成不同程度的脑外伤，致伤后将大鼠局部损伤的脑组织浸泡在人工海水或生理盐水中，构建动物模型。观察各组大鼠行为学、病理学、脑含水量、血脑屏障通透性等变化。结果 建立的动物模型可较好地模拟遭海水浸泡的开放性颅脑外伤伤情特点。随脑外伤程度的加重，大鼠神经功能损伤、脑组织病理学、脑含水量、血脑屏障破坏程度逐渐加重；同等伤情下海水浸泡组脑组织坏死和出血等病理改变较生理盐水浸泡组明显加重；浸泡后24 h，同等伤情下海水浸泡组较生理盐水浸泡组脑组织含水量、血脑屏障破坏程度等均有显著加重。结论 制作的大鼠模型可模拟不同程度开放性颅脑外伤遭海水浸泡后的伤情变化。 |
| 关键词: 开放性颅脑损伤 海水浸泡 动物模型 血脑屏障 |
| DOI：10.16781/j.0258-879x.2016.12.1538 |
| 投稿时间：2016-07-26修订日期：2016-11-06 |
| 基金项目:2014年度南京军区医学科技课题. |
|
| An animal model of open craniocerebral injury with seawater immersion |
| YANG Bing,LI Ming*,LIU Xin-long,XU Feng-feng,XU Zheng-ping |
(Department of Neurosurgery, No. 455 Hospital of PLA, Shanghai 200052, China
* Corresponding author) |
| Abstract: |
| Objective To establish an animal model of open craniocerebral injury with seawater immersion and to study the characteristics of the injury. Methods A total of 100 healthy male SD rats were divided into 5 groups:control group, mild injury+seawater immersion group, moderate injury+seawater immersion group, severe injury+seawater immersion group and severe injury+normal saline immersion group. After SD rats had received a controlled cortical impact (CCI) injury, the injured brain tissue was soaked in seawater or normal saline to construct an animal model of open craniocerebral injury. We then observed the characteristics of the model, including the animal behavior, pathological changes, brain water content and the level of blood brain barrier (BBB) opening. Results The animal model could well simulate the characteristics of the open craniocerebral injury with seawater immersion. With the increase of brain injury severity, the cerebral dysfunction, cerebral pathology, cerebral water content, and the damage of BBB became more serious. The pathological changes of brain tissue necrosis and bleeding in severe injury+seawater immersion group were more notable than those in the severe injury+normal saline immersion group. Compared with the severe injury+saline immersion group, the water content of brain tissue and the damage of BBB were significantly increased in the severe injury+seawater immersion group after immersion for 24 h. Conclusion The rat model we established in this study can simulate the open craniocerebral injury with seawater immersion. |
| Key words: open craniocerebral trauma seawater immersion animal models blood-brain barrier |
