| 摘要: |
| 视网膜新生血管性疾病是一类严重的致盲性疾病，构建与之相关的动物模型是进行该疾病机制研究的重要基础，有利于在临床中更好地预防、诊断和治疗。氧诱导视网膜病变小鼠模型是目前研究视网膜新生血管性疾病最常用的动物模型，此模型构建过程与人类早产儿视网膜新生血管病变的发生、发展极为相似，具有制作方法简单、重复性好等优点。本文重点阐述氧诱导视网膜病变小鼠模型的构建方法及原理，模型相关细胞因子的作用，模型构建的影响因素如动物品种、氧气的百分含量、高氧对母鼠的影响、产后体质量增加量、光照暴露等，并简要介绍模型中常用的量化评估方法，为研究人员准确、有效地构建和评估氧诱导视网膜病变小鼠模型提供帮助。 |
| 关键词: 早产儿视网膜病 新生血管化 氧诱导视网膜病变 动物模型 |
| DOI：10.16781/j.0258-879x.2017.11.1439 |
| 投稿时间：2017-03-08修订日期：2017-09-07 |
| 基金项目:国家自然科学基金（81470652）. |
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| Research advances in mouse model of oxygen-induced retinopathy |
| ZHAO Zi-chang,ZHAO Shi-hong* |
(Department of Ophthalmology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
*Corresponding author) |
| Abstract: |
| Retinal neovascular disease is a serious blinding disease. The construction of the related animal models is an important basis for conducting mechanism research and can contribute to better prevention, diagnosis and treatment of the disease. The mouse model of oxygen-induced retinopathy is the most commonly used animal model for studying retinal neovascular diseases, and its construction is similar to the development of retinopathy of prematurity, with the advantages of simple preparation method and good reproducibility. In this paper, we focused on the construction methods and principles of mouse models with oxygen-induced retinopathy, the role of model-related angiogenic cytokines, and factors influencing the construction of models (animal species, percentage of oxygen, effects of hyperoxia on maternal mice, postnatal weight gain, exposure of light and so on). Meanwhile, we briefly introduced the quantitative evaluation methods commonly used in the model, hoping to help researchers to accurately and effectively construct and evaluate the mouse model with oxygen-induced retinopathy. |
| Key words: retinopathy of prematurity neovascularization oxygen-induced retinopathy animal models |
