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| 利用原子力显微镜峰值力定量测量模式分析人间充质干细胞早期分化的力学表型 |
| 刘知晓1,2△,张金金1△,王鑫艳3,陈峰4,胡钧1,2,吕军鸿1,2* |
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(1. 中国科学院上海应用物理研究所物理生物学实验室, 上海 201800;
2. 中国科学院大学化学与化工学院无机化学教研室, 北京 100049;
3. 国家蛋白质科学中心(上海), 上海 200031;
4. 首都医科大学附属北京世纪坛医院, 北京 100038
△共同第一作者
*通信作者) |
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| 摘要: |
| 目的 建立通过力学表型来灵敏反映人间充质干细胞分化早期微小力学性质变化的技术。方法 使用基于原子力显微镜峰值力定量纳米力学作图（peak force quantitative nanomechanical mapping，PF-QNM）技术，测量在不同浓度氯化锂诱导下人间充质干细胞的力学变化过程。结果 4 mmol/L和30 mmol/L氯化锂处理干细胞48 h后其纳米力谱就有显著的差异；但要到72 h之后，平均杨氏模量才能区分不同浓度氯化锂处理所导致的差别。结论 纳米力谱比平均杨氏模量更能反映干细胞分化早期的力学性质变化。基于纳米力谱的力学表型可以作为物理生物学标记来鉴定干细胞的早期分化。 |
| 关键词: 间质干细胞 力学表型 纳米力谱 细胞分化 |
| DOI：10.16781/j.0258-879x.2017.10.1261 |
| 投稿时间：2017-04-27修订日期：2017-09-06 |
| 基金项目:国家自然科学基金（11474298，11405250），中国科学院前沿科学重点研究方向基金（QYZDJ-SSW-SLH019）. |
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| Mechanical phenotype during early differentiation of human mesenchymal stem cells by quantitative measurement of peak force in atomic force microscopy |
| LIU Zhi-xiao1,2△,ZHANG Jin-jin1△,WANG Xin-yan3,CHEN Feng4,HU Jun1,2,LÜ Jun-hong1,2* |
(1. Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China;
2. Department of Inorganic Chemistry, School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100049, China;
3. National Center for Protein Science Shanghai, Shanghai 200031, China;
4. Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China
△Co-first authors.
* Corresponding author) |
| Abstract: |
| Objective To establish a technique for sensitively reflecting the micro-mechanical changes of human mesenchymal stem cells (hMSCs) in their early differentiation process by mechanical phenotype. Methods Based on atomic force microscopy (AFM), peak force quantitative nanomechanical mapping (PF-QNM) technique was used to measure the nanomechanical spectroscopy of hMSCs during differentiation induced by different concentrations of lithium chloride. Results The nanomechanical spectroscopy of hMSCs induced by 4 mmol/L and 30 mmol/L lithium chloride was significantly different after 48 h, while there was significant difference in Young's modulus after 72 h. Conclusion Nanomechanical spectroscopy is superior to Young's modulus in reflecting the change of mechanical properties of hMSCs in their early differentiation phase. Mechanical phenotype based on nanomechanical spectroscopy can be used as a physical biomarker to identify the hMSCs at their early differentiation stage. |
| Key words: mesenchymal stem cells mechanical phenotype nanomechanical spectroscopy cell differentiation |
